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Advanced course in flow cytometry and cell sorting
Provider: Faculty of Health and Medical Sciences

Activity no.: 3216-17-00-00There are 151 available seats 
Enrollment deadline: 01/07/2017
Date and time30.08.2017, at: 09:00 - 01.09.2017, at: 17:30
Regular seats200
Course fee3,300.00 kr.
LecturersJan Pravsgaard Christensen
ECTS credits2.20
Contact personJan Pravsgaard Christensen    E-mail address:  jpc@sund.ku.dk
Enrolment Handling/Course OrganiserPhD administration     E-mail address: fak-phdkursus@sund.ku.dk

Aim and content
This course is free of charge for PhD students at Danish universities (except Copenhagen Business School). Special rules apply for research year students enrolled at Faculty of Health and Medical Sciences at UCPH. All other participants must pay the course fee.
Anyone can apply for the course, but if you are not a PhD student, you will be placed on the waiting list for the course until enrollment deadline. After the deadline of enrollment, available seats will be allocated to students on the waiting list.

Important information
When you have been approved for the course you need to register on the official site for the conference:http://www.flowcytometri.dk/ and check the box that you are on a course held by KU

Learning objectives
A student who has met the objectives of the course will be able to:

a) The student will get an in depth understand of protocols used in flow cytometry, demonstrated through lectures from people with experience in the field.
b) The student will get a better view of what flow cytometry can be used for, giving a better understanding on how they can use flow cytometry in their project.
c) The student will meet people with state-of-the-art knowledge in flow cytometry and related areas and get a chance to exchange knowledge with other students in the field.

Content
The course will start with a summer school, where you are introduced to basic and advanced flow cytometry. The summer school will take you through some of the pit falls in flow cytometry.
The course will then carry on as a series of state-of-the-art lectures from high end users in many fields in flow cytometry and cell sorting, this includes:
• Measurement of DNA using flow cytometry
• Measurement of RNA using flow cytometry
• Immunology, NK cells
• SWOFF analysis – the unrecognized sibling of FMO
• Multicolor flow cytometry
• Single cell genomics
• Functional assays
• Analysis and sorting of extracellular vesicles
• Bacteria, protists and plants in flow cytometry
• Clinical session, Studies of DNA ploidy and S-phase in endometriosis
• Clinical session, Immune deficiencies
• ImageStream applications
• Helios/CyTof applications
There will be 4 session dedicated oral presentations from students, and 2 poster viewing sessions in the afternoon, this will include:
• Clinical flow cytometry
• Research projects and cell sorting flow cytometry
• Research projects and flow cytometry
• Core facility management
It is mandatory for PhD students signed up at this course to submit an abstract.

Participants
PhD students and people on similar level, no previous skills in flow cytometry are needed, but limited knowledge in the field will be an advantage

Language
English

Form
State-of-the-art lectures, oral presentations in workshops and poster presentation.

Course director
Professor Jan Pravsgaard Christensen, ISIM, 35327873, jpc@sund.ku.dk

Teachers
High end users, that are experts in their fields, from around the world.

Course secretary
Jan P. Christensen
Department of Immunology and Microbiology
Blegdamsvej 3c, Build 22.5.12
Phone: 35327873
Mail: jpc@sund.ku.dk

Dates
30/8 – 1/9 2017

Course location
DGI-byen Tietgensgade 65, 1704 Copenhagen V

Registration: Please register before 1/7-2017

Admission to PhD students from Danish universities will be allocated on a first-come, first-served basis and according to the rules in force.
Applications from other participants will be considered after the last day of enrollment.

Program:Day 1 (Wednesday-August 30)
8:00-13:00 Registration
9:00-12:00 “Summer school” Mandatory for PhD students.
Advanced flow cytometry course (The complete meeting is offered as a PhD course in advanced flow cytometry by Copenhagen University)
(Rui Gardner, Memorial Sloan Kettering Cancer Center, USA, Andrew Filby, Newcastle University, UK and Karen Hogg, University of York, UK )
12:00-13:00 Lunch
13:00-13:10 Welcome by all three Scandinavian societies (plenum)
13:10-13:50 Session 1 (plenum) Measurement of DNA using flow cytometry
(Trond Stokke, Olso University Hospital, Norway)
13:50-14:30 Session 2 (plenum) Measurement of RNA using flow cytometry
(Rui Gardner, Memorial Sloan Kettering Cancer Center, USA)
14:30-15:00 Coffee in exhibition area
15:00-15:45 Session 3 (plenum) Immunology, NK cells
(Hans-Gustaf Ljunggren, Karolinska Institute, Sweden)
15:45-16:15 Session 4 (plenum) SWOFF analysis – the unrecognized sibling of FMO
(Michael Kapinsky, Beckman Coulter, Marseille, France)
16:30-17:30 Session 5 (plenum) Keynote speaker
(Pratip Chattopadhyay, NIH, US
17:30-18:30 Poster viewing
19:00-20:00 Canal tour in the Copenhagen canals


Day 2 (Thursday- August 31)
08:00-10:00 Registration
09:00-09:45 Session 6 (plenum) Single cell genomics
(Joshua Brickman, DanStem, Copenhagen, Denmark)
09:45-10:30 Session 7 (plenum) Functional assays
(Johannes Landskron, University of Oslo, Norway)
10:30-11:00 Coffee in exhibition area
11:00-12:30 2 parallel sessions (oral presentations selected from abstracts/posters, 15min)
A) Clinical flow cytometry
B) Research and cell sorting flow cytometry
12:30-13:30 Lunch
13:30-14:30 2 parallel sessions (oral presentations selected from abstracts/posters, 15min)
C) Research flow cytometry
D) Core facility management
14:30-15:00 Coffee
15:00-15:45 Session 8 (plenary). Analysis and sorting of extracellular vesicles
(Ger Arkesteijn, University of Utrecht, The Netherlands)
15:45-16:30 Session 9 (plenary). Bacteria, protists and plants in flow cytometry
(Karen Hogg, University of York, UK)
16:30-17:30 Poster session
18:30-23:00 Conference dinner (in the DGI building)



Day 3 (Friday- September 1st)
09:00-09:45 Session 10. Clinical session, Studies of DNA ploidy and S-phase in endometriosis
(Rasmus Green, Lund University, Sweden)
09:45-10:30 Session 11. Clinical session, Immune deficiencies
(Bjarne Kuno Møller, Aarhus University Hospital, Denmark)
10:30-11:00 Coffee in exhibition area
11:00-11:45 Session 12. ImageStream applications
(Andrew Filby, Newcastle University, UK)
11:45-12:30 Session 13. Helios/CyTof applications
(Vinko Tosevski, University of Zürich, Switzerland)
12:30-12:45 Closing remarks


Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students) If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.

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