This course will introduce to all important modalities of advanced biological and biomedical imaging using photons, electrons, X-rays and neutrons, available in the Øresund region in core facilities and at synchrotron and linear accelerators (MAX IV and ESS). These modalities offer a wide zoom range and the resolution power to resolve the substructure of molecules, cells, tissues, organs and whole bodies. The course is relevant for PhD students within biological, chemical, physical, medical, molecular and pharmaceutical sciences and will be coordinated with the MSc course "Biological Imaging".
In particular the following topics will be treated: fluorescence microscopy, fluorescence life time imaging, super resolution microscopy, electron and cryo electron microscopy, X-ray imaging and imaging at synchrotron and linac beamlines. An important part will be the concomitant data analysis, -management and –statistics as well as image processing.
This course aims at giving the student an understanding of biomedical imaging including the physical and optical principles of cutting-edge microscopes and beamlines. The course includes show cases at the instruments and, thus, will be an important asset for students that want to integrate biological and soft-matter imaging in their projects.After the course the student should be able to:Knowledge:Describe properties of different light sources and their impact on biological specimens;Understand the principles of specimen preparation;Process raw data from different imaging modalities, including multivariate analysis of 4D image series;Find the respective instruments in the Øresund region.Skills:Analyse and evaluate scientific papers which utilize biological imaging instruments and –beam lines;Make a flow-chart of data management from raw image data to analysis;Process image series with simple software algorithms for contrast and brightness improvement, segmentation and automated analysis.
Competences:Select appropriate imaging modalities to visualize molecular or cellular structures and processes;Describe the limitations of the different imaging modalities used in the course;Judge suitability of MAX IV or ESS to solve scientific questions of a project within Biology, Biology-Biotechnology, Computer Science, Medicine and Technology, Molecular Biomedicine, Nanoscience or Pharmaceutical Sciences while being aware of completing imaging instruments.
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