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Methods in Insect Pathology with Focus on entomopathogenic Fungi
Provider: Faculty of Science

Activity no.: 5695-20-09-31Activity has taken place
PlaceDepartment of Plant and Environmental Sciences
Date and timeSeptember 2019
Regular seats
ECTS credits4.00
Contact personNicolai Vitt Meyling    E-mail address: nvm@plen.ku.dk
Enrolment Handling/Course OrganiserNicolai Vitt Meyling    E-mail address: nvm@plen.ku.dk
Written languageEnglish
Teaching languageEnglish
Semester/BlockBlock 1 ¤ Block 2 ¤ Block 3 ¤ Block 4
Scheme groupNot included in the scheme group
Scheme group noteTo be decided
Exam formWritten assignment
Course workload
Course workload categoryHours
Practical exercises80.00
Project work30.00



Hands on introduction to methods used in insect pathology with focus on entomopathogenic fungi (Ascospherales, Entomophthorales and Hypocreales). The main areas include: a) Identification and taxonomy b) Isolation of entomopathogenic fungi from insects c) In vitro and in vivo cultivation d) Cryo-preservation of in vitro cultures of entomopathogenic fungi for long term storage e) Bioassay techniques of entomopathogenic fungi, including estimation of dose, analysis of data.

The course covers theoretical and practical aspects of identification and taxonomy of Ascospherales, Entomophthorales and Hypocreales. This part includes preparation of sexual and asexual spore slides (Ascomata, resting spore and conidia), brief introduction to mycological terms used for classification and identification. Training in identification will be performed on class material covering the most common genera of insectpathogenic fungi and include different staining methods.

The course will give an introduction to in vitro growth of entomopathogenic fungi (in liquid and solid media). Furthermore, the course will cover techniques for in vitro isolation of entomopathogenic fungi from diseased insects followed by Cryo-preservation of in vitro cultures at -80 degrees of entomopathogenic fungi for long term storage.

Bioassay techniques of entomopathogenic fungi will cover the most commonly used assay techniques (per os, conidia shower or dipping) including spore concentration preparations and spore germination tests. Further, methods for estimation of doses and data recording will be included. Finally, data analysis will be discussed and various statistical packages will be presented.


Bissett, J. (1987): Contribution toward a monograph of the genus Ascosphaera. Can. J. Bot. 66: 2541-60.

Hajek, A. E. Papierok, B. and Eilenberg, J. (2012): Methods for study of the Entomophthorales. In.: Lacey, L. (ed.) Manual of Techniques in Insect Pathology pp. 285-315. Academic Press, San Diego, USA.

Humber, R. A. (2007): Recent phylogenetically based reclassifications of fungal pathogens of invertebrates. http://www.sipweb.org/fungi/humber.pdf

Humber, R. A. (2012): Identification of entomopathogenic fungi. In.: Lacey, L. (ed.), Manual of Techniques in Insect Pathology pp. 151-186. Academic Press, San Diego, USA.

Humber, R. A. (2012): Preservation of entomopathogenic fungal cultures. In.: Lacey, L. (ed.), Manual of Techniques in Insect Pathology pp. 317- 327. Academic Press, San Diego, USA.

Inglis, G. D., Enkerli, J. and Goettel, M. S. (2012) Laboratory technuiqes used for entomopathogenic fungi: Hypocreales. In.: Lacey, L. (ed.) Manual of Techniques in Insect Pathology pp. 189-215. Academic Press, San Diego, USA.

Jensen A. B., Aronstein K., Flores J. M., Vojvodic S., Palacio A., Spivak M. (2012). Methods for handling of fungal brood diseases – chalkbrood caused by Ascosphaera apis and stonebrood caused by Aspergillus spp. Journal of apicultural Research 52(1) DOI 10.3896/IBRA.

Teaching and learning methods
Laboratory based; individually arranged.

Annette Bruun Jensen

Please contact course responsible Nicolai Vitt Meyling if you are interested in attending the course.

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