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Introduction to Nutritional Metabolomics
Provider: Faculty of Science

Activity no.: 5472-18-06-31There are no available seats
Enrollment deadline: 01/02/2018
PlaceDepartment of Nutrition Exercise and Sports
Date and time26.02.2018, at: 08:00 - 09.03.2018, at: 16:00
Regular seats4
Course fee0.00 €
ECTS credits4.00
Contact personGözde Gürdeniz    E-mail address: gozde.gurdeniz@food.ku.dk
Enrolment Handling/Course OrganiserLars Ove Dragsted    E-mail address: LDRA@NEXS.KU.DK
Written languageEnglish
Teaching languageEnglish
Semester/BlockBlock 3
Block noteDuration: 6 days
Exam requirementsOral exam
Exam formOral defence, presentation of the Group Work, Critical questions from lecturers
Exam aidsWithout aids
Grading scalePassed / Not passed
Criteria for exam assessmentContinuous participation and oral presentation of the group work.
Course workload
Course workload categoryHours
Preparation25.00
Lectures25.00
Project work25.00
Theory exercises25.00
Sum100.00
Content
The course will provide an overview on LC-MS based untargeted metabolomics and its application in nutrition. It will be delivered using a mixture of lectures, hands-on sample preparation and analysis, computer-based practical sessions and discussions.

The students will go through common steps in a typical metabolomics study using an example study. The example study will be collected plasma (or urine) samples from a nutritional intervention. They will work hands-on with sample preparation and analysis on UPLC-QTOF and further process and analyze the acquired data with various free tools (Workflow4Metabolomics and MetaboAnalyst). They will finally work on identification of relevant metabolites using several web-based structure elucidation tools. The course will finalize by presentations of reports generated by the students based on the case study.

The course will be structured as initial short lectures on theory followed by hands-on exercises which will let the students to transfer the theoretical information to practice.

Learning outcome
The aim of this course is to introduce the students to all phases in a nutritional metabolomics study and to train the student in the sample preparation and analysis and use of freely available tools for data handling.

After completing the course the students should be able to:

• To prepare the urine and plasma samples for metabolomics analysis
• Understand the basic principles of UPLC-QTOF technology and get familiar with sample analysis
• Suggest the sample type to analyze for a specific research question and propose the relevant sample collection and preparation procedure
• Carry out data preprocessing using freely available tools (XCMS, Workflow4Metabolomics)
• Perform basic univariate and multivariate analysis (MetaboAnalyst)
• Interpret the MS/MS spectra by utilizing available tools (e.g. CAMERA, MetFusion, MetFrag) and databases (e.g. HMDB, MassBank, MZCloud)

Teaching and learning methods
Lectures, hands-on exercises, group discussions.

Lecturers
Assistant Professor Gözde Gürdeniz
Professor Lars O. Dragsted
PhD student Natalia Vazquez Manjarrez
PhD student Catalina Cuparencu

Remarks
Course responsibles:

Lars Ove Dragsted (ldra@nexs.ku.dk)
Gözde Gürdeniz (gozg@nexs.ku.dk)

Date of the exam is Friday 23 March 2018 from 09:00 to 10:30.

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