Faculty of Science

Introduction to Nutritional Metabolomics
Provider: Faculty of Science

Activity no.: 5472-20-06-31
	Enrollment deadline: 03/08/2020

Place

Department of Nutrition Exercise and Sports

Date and time

17.08.2020, at: 09:00 - 21.08.2020, at: 16:00

Regular seats

Course fee

300.00 €

ECTS credits

3.00

Contact person

Jan Stanstrup E-mail address: JST@NEXS.KU.DK

Enrolment Handling/Course Organiser

Lars Ove Dragsted E-mail address: LDRA@NEXS.KU.DK

Written language

English

Teaching language

English

Semester/Block

Summer

Block note

Duration: 41/2 days

Exam form

Continuous assessment

Exam details

Oral defence, presentation of the Group Work, critical questions from lecturers

Grading scale

Passed / Not passed

Course workload

Course workload category	Hours
Preparation	40.00
Lectures	18.00
Project work	14.00
Theory exercises	6.00

Sum	78.00

Content

The course will provide an overview of LC-MS based untargeted metabolomics and its application in nutrition. It will be delivered using a mixture of lectures, hands-on data preparation and analysis, computer-based practical sessions, and discussions. Visits to wet labs and instructions on human sample preparation procedures is included but with minimal hands-on.

The students will go through common steps in a typical metabolomics study using a real-life case. This case study includes collected plasma (or urine) samples from a nutritional intervention. The sample preparation and analysis on UPLC-QTOF has been conducted and the students will further process and analyse the acquired data with various freeware tools (e.g. R, XCMS, MZmine and Metaboanalyst). They will finally work on identification of relevant metabolites using several web-based structure elucidation tools. The course will conclude by presentations of reports generated by the students based on the case study.

The course will be structured as initial short lectures on theory followed by hands-on exercises, which will teach the students to transfer the theoretical information to practice.

Learning outcome

The aim of this course is to introduce the students to all phases in a nutritional metabolomics study, to instruct the student on sample handling, and to train the student in data analysis and in the use of freely available tools for the metabolomics data flow.

After completing the course the students should be able to:

• Handle urine, plasma and other samples collected from humans for metabolomics analysis
• Understand the basic principles of UPLC-QTOF technology and get familiar with sample analysis
• Suggest which sample type to analyze for a specific research question and propose the relevant sample collection and preparation procedure
• Carry out data preprocessing using freely available tools (R/XCMS)
• Perform basic univariate and multivariate analysis (e.g. R, MetaboAnalyst)
• Interpret the MS/MS spectra by manual interpretation and by utilizing available tools (e.g. MetFusion, MetFrag) and databases (e.g. HMDB, METLIN and MassBank)

Teaching and learning methods

Lectures, hands-on exercises, group discussions.

Lecturers

Professor Lars Ove Dragsted
Assistant Professor Jan Stanstrup
Assistant Professor Cristian de Gobba
Assistant Professor Henrik Munch Roager
Post doc Giorgia La Barbera
PhD student Ceyda Pekmez
PhD student Muyao Xi

Remarks

Location
Rolighedsvej 26, rooms A001 and A009

Fee
No fee for the PhD students under the Open Market in Denmark and NOVA partners (Nordic Countries).
Other participants are to pay a course fee of 300 EUR. Each student must pay and arrange their own travel and accommodation in Copenhagen during the course.

The program can be downloaded here.

For more information please contact Jan Stanstrup: jst@nexs.ku.dk.

Date of the exam is Friday 10 July 2020 from 09:00 to 12:00.

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