Sample Preparation and Separation Techniques in Bio-Analytical Chemistry
Provider: Faculty of Health and Medical Sciences

Activity no.: 3130-21-00-00 
Enrollment deadline: 01/08/2021
Date and time06.09.2021, at: 09:00 - 17.09.2021, at: 16:00
Regular seats14
Course fee14,160.00 kr.
LecturersNickolaj Petersen
ECTS credits5.70
Contact personMarianne Wieslander Jørgensen    E-mail address:
Enrolment Handling/Course OrganiserPhD administration     E-mail address:

Aim and content
This course is free of charge for PhD students at Danish universities (except Copenhagen Business School), and for PhD students at graduate schools in the other Nordic countries. All other participants must pay the course fee.

Anyone can apply for the course, but if you are not a PhD student at a Danish university, you will be placed on the waiting list until enrollment deadline. This also applies to PhD students from Nordic countries. After the enrollment deadline, available seats will be allocated to applicants on the waiting list.

Learning objectives
A student who has met the objectives of the course will be able to:

1. Develop and design methods for analysis of biological samples (e.g. urine and plasma) using advanced analytical chemical techniques

2. Master a broad selection of modern separation and detection techniques as well as sample preparation techniques used for the analysis of biological samples

3. Know about analytical chemical scientific literature and relevant data bases

4. Have practical experience with HPLC method development, ionpair chromatography, protein precipitation, GC-MS for analysis of biological samples, chiral capillary electrophoresis

The course objectives are to illustrate the use of separation methods in drug analysis and bio-analysis. Topics will be separation mechanisms in gas chromatography, high-performance liquid chromatography, super critical chromatography and capillary electrophoresis and the choice of separation technique for a given bio-analytical problem. Various techniques for sample preparation will be discussed in order to provide participants with sufficient knowledge to be able to develop bio-analytical methods efficiently.

One half of the course will be devoted to the discussion of different separation techniques in chromatography and electrophoresis (gas chromatography, high-performance liquid chromatography, super critical chromatography and capillary electrophoresis), and the advantages and limitations of each technique will be discussed in detail. The course will focus on separation mechanisms and on the right choice of separation technique for different bio-analytical problems as well as problems in drug analysis.

The other half of the course will deal with sample preparation and with detection principles as these two parts of an analytical method depends on one another, thus being most important in the selection of the final method. Finally, method validation will be discussed.

- Chromatographic theory: The partitioning process; the van Deemter plot; the capacity factor, resolution, efficiency and selectivity.

- Separation mechanism: Stationary phase, mobile phase and buffer systems; column packing materials for chromatographic techniques; capillary technology for capillary electrophoresis; chiral separations.

- Quantitative analysis, qualitative analysis and trace analysis.

- Equipment: Mobile phase delivery; sample introduction systems; detection systems; data collection systems; automation.

- Detection: Spectroscopic methods (UV, IR, NMR, MS, diode array, fluorescence); amperometric; radiochemical; derivatisation.

- Sample preparation: Liquid-liquid extraction; solid phase extraction; dialysis; protein precipitation; on-line derivatisation; on-line sample preparation.

- Method validation.

The course is relevant for all students performing or who is planning to perform analytical chemical analysis in the biosciences. A basic knowledge within chemistry and physical chemistry as well as spectroscopy is an advantage.

Relevance to graduate programmes
The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH:

All graduate programmes

The course will normally be taught in English. However, if only Danish participants are enrolled the course will be taught in Danish.

The course consists of about 25 hours of lectures/seminars and 20 hours of laboratory work. The course will run each day during two weeks. The first week lectures/seminars will take place from 9 to 12 o'clock in the morning. In the second week lectures will be held from 9 to 11 o'clock in the morning and laboratory work will take place 4 hours each afternoon.

The course will be concluded by a four hour written examination, which will be assessed by the course director. All printed and written material as well as electronic calculators are allowed.

Course director
Nickolaj J. Petersen, Associate Professor, Department of Pharmacy, University of Copenhagen.

Stig Pedersen-Bjergaard, Professor, Department of Pharmacy
Jörg Kutter, Professor, Department of Pharmacy
Kasper Dyrberg Rand, Professor, Department of Pharmacy
Claus Cornett, Associate Professor, Department of Pharmacy

6 - 17 September 2021 and a written exam in November

Course location
PharmaSchool, Universitetsparken 2, 2100 Copenhagen.

Please register before 1 August 2021

Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules.
Applications from other participants will be considered after the last day of enrolment.

Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.