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BIO Advanced Live Cell Imaging
Provider: Department of Biology

Activity no.: 5095-22-01-31 
Enrollment deadline: 06/05/2022
Date and time27.06.2022, at: 09:00 - 01.07.2022, at: 16:00
Regular seats25
ECTS credits5.00
Contact personNynne Christensen    E-mail address: nmchristensen@bio.ku.dk
Enrolment Handling/Course OrganiserNynne Christensen    E-mail address: nmchristensen@bio.ku.dk
Written languageEnglish
Teaching languageEnglish
Exam formCompleted/not completed
Exam detailsPreparation of an abstract and a presentation for the Bioimaging Workshop Copenhagen
Course workload
Course workload categoryHours
Preparation / Self-Study50.00
Course hours37.00
Evaluation / reporting40.00


The summer course for experienced microscopists comprises theory and show-cases on selected topics, such as Fluorescence Lifetime, Super Resolution and High Throughput Microscopy as well as high speed live and spectral imaging. Internationally outstanding experts will give key notes on these topics. It will be possible for the students to bring their own specimens, if suitable for the chosen techniques and agreed upon (please indicate your interest at the latest end of May). Part of the summer course is the 8th Bioimaging Workshop Copenhagen with opportunity to present posters or talks.

Aim and content
Monday, June 27 Morning: Introduction (Alexander Schulz) Theory 1: Microscopy basics, theory of fluorescence (Alexander Schulz) 4x 10min participant presentations Theory 2: Molecular biology and microscopy, Visualization of protein-protein interaction with Bimolecular fluorescence complementation (Michael Lisby) Afternoon: 4x 10min participant presentations Theory 3: Cell physiology, live-cell imaging (Ivana Novak) 4x 10min participant presentations Theory 4: Introduction to advanced optical instruments, super-resolution and spinning-disc microscopy (Sebastian Kjeldgaard-Nintemann and Nynne Christensen) 4x 10min student presentations Evening: Reception

Tuesday, June 28 Morning: 1. Guest lecture by Michael Kühl: Sensor imaging 2. Lecture by Felicity Mae Davis : Imaging activity across scales in the mammary gland. Afternoon: Show-case: Super-resolution microscopy (Alexander Schulz, Nynne Christensen)Showcase: Protein-protein interaction, Fluorescence lifetime imaging (Sebastian Kjeldgaard-Nintemann), Alexander Schulz, Nynne Christensen,)

Wednesday, June 29 Morning:1. Guest lecture by Kalina Hass: super resolution 2. Guest lecture by Dorus Gadella: FLIM - FRET Afternoon: Hands-on: High content screening of living yeast cells (Michael Lisby, Nynne Christensen) Hands-on: confocal and two-photon microscopy ( Sebastian Kjeldgaard-Nintemann), Ivana Novak, Nynne Christensen, Alexander Schulz)

Thursday, June 30 Morning: Theory 5: AI and machine learning (Michael Lisby, Selvan Raghavendra) Hands-on: Image analysis (everybody) Afternoon: Bioimaging Workshop Evening: Bioimaging Workshop dinner

Friday, July 1 Morning: Bioimaging Workshop Afternoon: Discussion and course conclusion

Formel requirements
Students interested in the course are requested to apply with a short motivation and, when accepted, to give a short presentation of their research project and make an oral presentation or a poster at the Bioimaging Workshop.

Learning outcome
Participants will learn the theory behind advanced microscopy techniques and how to utilize their full potential from internationally outstanding experts that will be part of the course. In addition there will be a session on image processing and analysis.

Target group
PhD students within Life Sciences

Teaching and learning methods
Keynote lectures given by internationally outstanding experts, lectures, show-cases at the instruments, flipped classroom for image processing and analysis and preparation of an abstract for the workshop

Guest lecturer:

Thomas Nyström.
Department of Immunology and Microbiology, Institute of Biomedicine, Sahlgrenska Academy
University of Gothenburg, Sweden

Jan Huisken
Humboldt Professor of Multiscale Biology, Georg-August-University Göttingen, Germany

Kalina Hass
Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, Versailles, France.

Theodorus Gadella
University of Amsterdam. Director of the van Leeuwenhoek Centre for Advanced Microscopy. Chairman of the section of Molecular Cytology.

Felicity Mae Davis
Department of Biomedicine, Health, Aarhus University, DK

Professor Ivana Novak, inovak@bio.ku.dk
Professor Michael Lisby, mlisby@bio.ku.dk
Professor Alexander Schulz,als@plen.ku.dk

Nynne Christensen, nmchristensen@bio.ku.dk
Sebastian Kjeldgaard-Nintemann, seni@plen.ku.dk

Professor Michael Kûhl (BIO)
Assistent professor Raghavendra Selvan (DIKU)

Signing Up:

If you would like to participate please:
1) Register online by clicking on "Apply" and
2) Send an e-mail to Nynne M Christensen stating your motivations (10 lines) and which microscopy methods you have used before (2-3 lines).

The course takes place at the Center for Advanced Imaging with locations at the North Campus and the Frederiksberg Campus.

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