Faculty of Science

Advanced Methods in Light Microscopy
Provider: Faculty of Science

Activity no.: 5729-24-09-31
	Enrollment deadline: 16/08/2024

Place

Department of Plant and Environmental Sciences

Date and time

16.09.2024, at: 00:00 - 27.09.2024, at: 16:00

Regular seats

Course fee

750.00 kr.

ECTS credits

4.00

Contact person

Alexander Schulz E-mail address: als@plen.ku.dk

Enrolment Handling/Course Organiser

Alexander Schulz E-mail address: als@plen.ku.dk

Written language

English

Teaching language

English

Scheme group

Not included in the scheme group

Exam form

Course Participation; Written Assignment

Exam details

Written report documenting the results of the hands-on practicals

Grading scale

Approved / Not approved

Course workload

Course workload category	Hours
Lectures	20.00
Project work	20.00
Practical exercises	20.00
Guidance	10.00
Preparation	50.00

Sum	120.00

Content

The aim of the course is to introduce PhD students to the professional use of research microscopes.
The teachers are members of the Center for Advanced Bioimaging (CAB) Denmark. Where relevant, preparation methods for the respective microscope type and hands-on at the instruments are included.
The course covers various light microscopic methods (bright field, phase contrast, fluorescence, polarisation, differential contrast).
The optical and technical background is thoroughly discussed (resolution, Köhler illumination, wave optics, diffraction, amplitude and phase specimens, birefringence), and measuring methods and the effects of different instrument settings are practiced. Please note that confocal microscopy will be theoretically introduced, but that training at the instrument is not part of this course.
The participants are requested to come with their own specimens and will learn how these can optimally be imaged and documented.

Learning outcome

>Understanding the optical principals used in light microscopy
>Understanding the background of super-resolution microscopy
>Handling and optimal setting of different research microscopes
>Working with simple vital staining methods
>Optimising digital camera settings for micrographs
>Using image processing and analysis software

Literature

Course manual (distributed before the start of the course)
Text book (for students planning to use microscopy as a major source of their project)
Douglas M Murphy and Michael W Davidson (2013) Fundamentals of Light Microscopy and Electgronic Imaging, Wiley- Blackwell

Target group

PhD strudents within Life and Biomedical Sciences

Teaching and learning methods

Lectures, hands-on microscopes, journal club presentations and discussions, working in 2-person teams

Remarks

Signing Up:
If you would like to participate please:

1) Register online by clicking on "Apply" and
2) Send an e-mail to Alexander Schulz stating your motivations (10 lines) and which microscopy methods you have used before (2-3 lines).

There is a course fee for participants of 750 DKK to cover lab costs
*
UCPH discloses non-sensitive personal data to course leader/speakers, if any. In addition, we will disclose non-sensitive personal data to the other participants in the course. Non-sensitive personal data includes names, job positions, institution names & addresses, telephone numbers and e-mail addresses.

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