BIO Advanced Live Cell Imaging
Provider: Faculty of Science

Activity no.: 5095-24-01-31 
Enrollment deadline: 10/06/2024
PlaceCenter for Advanced Imaging with locations at the North Camp
and the Frederiksberg Campus
Date and time21.06.2024, at: 09:00 - 27.06.2024, at: 16:00
Regular seats25
ECTS credits5.00
Contact personNynne Christensen    E-mail address: nmchristensen@bio.ku.dk
Enrolment Handling/Course OrganiserNynne Christensen    E-mail address: nmchristensen@bio.ku.dk
Written languageEnglish
Teaching languageEnglish
Exam formCompleted/not completed
Exam detailsPreparation of a flash talk for the first course day, and a poster or talk on the DBI meeting
Course workload
Course workload categoryHours
Preparation / Self-Study102.00
Lectures12.00
Laboratory8.00
Practical exercises16.00
Evaluation40.00
Sum178.00
Content
The summer course for experienced microscopists comprises theory and show-cases on selected topics, such as Fluorescence Lifetime, Super Resolution and High Throughput Microscopy as well as high speed live and spectral imaging. Internationally outstanding experts will give key notes on these topics. It will be possible for the students to bring their own specimens, if suitable for the chosen techniques and agreed upon (please indicate your interest at the latest end of May). Part of the summer course is the annual meeting of the Danish Bioimaging network with opportunity to present posters or talks.

Aim and content

Friday, June 21 Morning: Introduction (Alexander Schulz) Theory 1: Microscopy basics, theory of fluorescence (Alexander Schulz) 4x 10min participant presentations Theory 2: Molecular biology and microscopy, Visualization of protein-protein interaction with Bimolecular fluorescence complementation (Michael Lisby) Afternoon: 4x 10min participant presentations Theory 3: Cell physiology, live-cell imaging (Jakob Grunnet Knudsen) 4x 10min participant presentations Theory 4: Introduction to advanced optical instruments, super-resolution and spinning-disc microscopy (Sebastian Kjeldgaard-Nintemann and Nynne Christensen) 4x 10min student presentations Evening: Reception

Monday, June 24: Danish Bioimaging network meeting at Panum, scientific talks and workshops, Conference dinner. Please sign up here: DBI Scientific Meeting | Sign up (ku.dk)

Tuesday, June 25: Danish Bioimaging network meeting at Panum, scientific talks + workshops

Wednesday June 26, Morning: 1. Guest lecture by Roland Nitschke: Quality assessment and reproducibility for instruments and images in light microscopy. 2. Guest lecture by Dorus Gadella: FLIM/FRET. Afternoon: Show-case: High content screening microscopy (Nynne Christensen) Showcase: Confocal imaging, Fluorescence lifetime imaging (Sebastian Kjeldgaard-Nintemann),

Thursday June 27:,Morning: 1. Guest lecture by Lecture by Julia Fernandez-Rodrigue: Correlated Multimodal Imaging 2. Guest lecture to be determined. Afternoon: Hands-on: Spinning disk confocal imaging (Guillermo Sergio Moreno Pescador) Hands-on: Perfussion setup, live imaging of tissue (Jakob Grunnet Knudsen). Discussion and course conclusion.

Formel requirements
Students interested in the course are requested to apply with a short motivation and, when accepted, to give a short presentation of their research project and make an oral presentation or a poster at the DBN meeting

Learning outcome
Participants will learn the theory behind advanced microscopy techniques and how to utilize their full potential from internationally outstanding experts that will be part of the course. It will be possible for the students to bring their own specimens.
The course will enable the participants to design bioimaging experiments for their own PhD project.

Target group
PhD students within the biological, biophysical, environmental, life, and medical sciences.

Lecturers
Guest lecturer:
Julia Fernandez-Rodriguez, University of Gothenburg, will give a talk on Correlated Multimodal Imaging and participate in the hands-on laboratory exercise.

Dorus Gadella, University of Amsterdam, will give a talk on fluorescence life-time imaging microscopy and participate in the hands-on laboratory exercise.

Roland Nitschke, University of Freiburg, will give a talk on Quality assessment and reproducibility for instruments and images in light microscopy and participate in the hands-on laboratory exercise

From UCPH-CAB:
Professor Michael Lisby, mlisby@bio.ku.dk
Professor Alexander Schulz,als@plen.ku.dk
Assistant Professor Jakob Grunnet Knudsen, jgknudsen@bio.ku.dk

Nynne Christensen, nmchristensen@bio.ku.dk
Sebastian Kjeldgaard-Nintemann, seni@plen.ku.dk

Workload

 

Remarks
Signing up:
If you would like to participate please:
1) Register online by clicking on "Apply" and
2) Send an e-mail to Nynne M Christensen stating your motivations (10 lines) and which microscopy methods you have used before (2-3 lines).

The course takes place at the Center for Advanced Imaging with locations at the North Campus and the Frederiksberg Campus.