Faculty of Science

Practical Multidimensional Chromatography
Provider: Faculty of Science

Activity no.: 5707-25-09-31
	Enrollment deadline: 10/11/2024

Place

Department of Plant and Environmental Sciences

Date and time

09.11.2024, at: 00:00 - 13.11.2024, at: 16:00

Regular seats

ECTS credits

2.50

Contact person

Nikoline Juul Nielsen E-mail address: njn@plen.ku.dk

Enrolment Handling/Course Organiser

Jan H. Christensen E-mail address: jch@plen.ku.dk

Written language

English

Teaching language

English

Semester/Block

Block 2

Exam form

Oral examination

Grading scale

Passed / Not passed

Course workload

Course workload category	Hours
Preparation	31.75
Theoretical exercises	4.00
Practical exercises	7.00
Laboratory	6.00
Lectures	20.00

Sum	68.75

Enrolment guidelines

Students will be introduced to the fundamental concepts and merits of multidimensional separation techniques encompassing GC, LC, and SFC. Following hand on laboratory sessions, analyzing samples using pulsed-elution and comprehensive LC×LC plus comprehensive GC×GC, students will gain practical experience in the most common data analysis ad chemometric strategies in two-dimensional chromatography.
The course objectives are to illustrate the advantages and necessity of using comprehensive two-dimensional chromatographic techniques in the analysis of complex samples.
Lectures will be given on the history and methodology of comprehensive multi-dimensional separations using gas (GC), liquid (LC), and supercritical fluid chromatography (SFC). Applications, method development and data analysis will be covered in detail, followed by practical lab-work where students will gain hands-on experience in both commercial and in-house data analysis software, gaining insights into their previously acquired data.

Formal requirements

The participants of the course are required to have a background in chromatography with a theoretical and practical understanding of one dimensional techniques.

Learning outcome

Knowledge:
•Understand the history, operating principles and applications of multidimensional separations encompassing GC, LC, and SFC
•Understand theoretical principles of multidimensional chromatography.

Skills:
•Carry out method development in LC×LC and thermally modulated GC×GC.
•Analyze samples using pulsed elution and comprehensive LC×LC-MS and thermally modulated GC×GC-MS

Competences:
•Make meaningful conclusions from the multidimensional data acquired using both commercial and in-house data analysis software.
•Be able to evaluate a separation problem and have the knowledge to select a multidimensional techniques suitable for solving the problem at hand.

Target group

The target group is PhD students from primarily Danish universities with the possibility also for international PhD students to join. The students are expected to have a background within analytical chemistry and motivated to learn about multidimensional techniques to solve challenges with identifying compounds in their own research.
The course aims to attract students from different scientific fields: environmental, biological and pharmaceutical research. In this way, we encourage networking and forming of a multidimensional community in Denmark with international links.

Teaching and learning methods

The course will be a mixture of lectures, laboratory exercises as well as theoretical and practical data processing exercises.

Lecturers

André de Villiers, University of Stellenbosch, South Africa

Karine Faure, Institut of Analytical Sciences, University of Lyon, France

Peter Q. Tranchida,University of Messina, Italy

Remarks

The course is free of charge for PhD students.

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Contact persons:
Jason Patrick Devers, jpd@plen.ku.dk
Oskar Munk Kronik omkr@plen.ku.dk
Nikoline J Nielsen njn@plen.ku.dk
Giorgio Tomasi gito@plen.ku.dk
Jan H. Christensen (prof.), jch@plen.ku.dk
Giorgio Tomasi (assoc. prof.) gito@plen.ku.dk
Nikoline J. Nielsen (assoc. prof.) njn@plen.ku.dk

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