Faculty of Science

Summer school in Advanced Live Cell Imaging
Provider: Faculty of Science

Activity no.: 5133-26-00-00	There are 25 available seats
	Enrollment deadline: 06/03/2026

Date and time	04.05.2026, at: 09:59 - 08.05.2026, at: 16:00
Regular seats	25
Lecturers	Michael Lisby
ECTS credits	2.50

Contact person	Michael Lisby E-mail address: mlisby@bio.ku.dk

Enrolment Handling/Course Organiser	PhD Administration SCIENCE E-mail address: phdcourses@science.ku.dk

Enrolment guidelines This is a specialised course where 50% of the seats are reserved for PhD students enrolled at the Faculty of SCIENCE at UCPH and 50% of the seats are reserved for PhD students at other faculties and universities. Seats will be allocated on a first-come, first-served basis and according to the applicable rules. Anyone can apply for the course, but if you are not a PhD student, you will be placed on the waiting list until enrollment deadline. After the enrollment deadline, available seats will be allocated to applicants on the waiting list. *Requirements for signing up - Special rules apply for this course* Please note that all applicants will be placed on the waiting list upon registration. After registering for the course, please send a motivational letter to the course coordinator by e-mail latest on 06 March 2026. About one week after registration deadline, all applicants on the waiting list will be notified whether they are given a seat. Course coordinator: Professor Michael Lisby, Department of Biology, mlisby@bio.ku.dk Aim and Content Monday, May 4 June Morning: Introduction (Alexander Schulz) Theory 1: Microscopy basics, theory of fluorescence (Alexander Schulz) 4x 10min participant presentations Theory 2: Molecular biology and microscopy, Visualization of protein-protein interaction with Bimolecular fluorescence complementation (Michael Lisby) Afternoon: 4x 10min participant presentations Theory 3: Cell physiology, live-cell imaging (Jakob Grunnet Knudsen) 4x 10min participant presentations Theory 4: Introduction to advanced optical instruments, confocal, multiphoton and ramen microscopy (Sebastian Kjeldgaard-Nintemann and Nynne Christensen) 4x 10min student presentations Evening: Reception Tuesday, May 5: Morning: Guest lecture by Silvia Barabino: laser microirradiation. Afternoon: Showcase: High content screening microscopy (Nynne Christensen) Showcase: Confocal and spectral scanning microscopy (Sebastian Kjeldgaard-Nintemann), show-case: Spinning disk confocal imaging (Guillermo Sergio Moreno Pescador) Wednesday May 6: Morning: Guest lecture by Giulio Nicola Cerullo: Ramen microscopy. Afternoon: Showcase: Multiphoton and fluorescence lifetime imaging (Nynne M Christensen), Showcase: Ramen Microscopy ((Sebastian Kjeldgaard-Nintemann). Tour to the experimental microscopy setup (Guillermo Sergio Moreno Pescador). Preparation for Danish Bioimaging meeting. Thursday May 7: Danish BioImaging Symposium at Danish Cancer Institute, scientific talks and workshops, Conference dinner. Friday May 8: Guest lecture by Judith Mine-Hattab: superresolution imaging. Danish BioImaging Symposium at Danish Cancer Institute, scientific talks + workshops Scientific content Theory of and hands-on experience with fluorescence microscopy techniques such as bimolecular fluorescence complementation, laser microirradiation, super-resolution, Ramen microscopy and spectral scanning microscopy, spinning-disc microscopy, fluorescence lifetime imaging, high-throughput microscopy-based screening, and image analysis and data management. Experimental considerations relating to cell physiology and phototoxicity during live-cell imaging will be taught and discussed. Teaching and learning methods Lectures in the morning by invited leading experts followed in the afternoon with show-cases on the advanced microscopes tying the theory and practical operation together. The last two days of the course the students join the Danish BioImaging Symposium held at the Danish Cancer Institute, a scientific conference that brings together the Danish bioimaging network, allowing the students to network with Danish and international experts of microscopy methods and present their data in either a poster or a short talk. Learning outcome Participants will learn the theory behind advanced microscopy techniques and how to utilize their full potential from internationally outstanding experts that will be part of the course. It will be possible for the students to bring their own specimens. The course will enable the participants to design bioimaging experiments for their own PhD project. Knowledge: • Of advanced techniques in microscopy, such as bimolecular fluorescence complementation, laser microirradiation, super-resolution, Ramen microscopy and spectral scanning microscopy, spinning-disc microscopy, fluorescence lifetime imaging, and high-throughput microscopy-based screening. • Of sample preparation Skills: • To operate four of the showcased microscopes • To present own research project Competences: • To design microscopy experiments Target Group PhD students within the biological, biophysical, environmental, life, and medical sciences Recommended Academic Qualifications A MSc degree or equivalent within the biological, biophysical, environmental, life, or medical sciences. Students with prior experience with basic microscopy are preferred. Students are requested to apply with a short motivation and, when accepted, to give a short presentation of their research project and make an oral presentation or a poster at the Danish Bioimaging Symposium. Research Area Biological, biophysical, environmental, life science, and biomedical sciences. Teaching and Learning Methods Keynote lectures given by internationally outstanding experts, lectures, show-cases at the instruments, flash talk and poster presentation by each student Type of Assessment Students have to give a short presentation of their research project and make an oral presentation or a poster at the Danish BioImaging Symposium. Literature Lab manuals. Six primary articles related to the showcases. Course coordinator Professor Michael Lisby, Department of Biology, mlisby@bio.ku.dk Guest Lecturers Silvia M L Barabino, Department of Biotechnology and Biosciences. Topic: laser microirradiation to study biocondensates) Judith Mine-Hattab, Sorbonne Paris, France. Topic: Single-particle tracking (SPT) experiments on DNA damage response proteins and condensates in vivo) Giulio Cerullo, Politecnico di Milano, Italy. Topic Ramen scanning microscopy Poul Martin Bendix, NBI, KU. Topic: Optical tweezers. Dates 4-8 May 2026 Expected frequency Biannually. Course location Copenhagen Plant Science Center (PLEN), August Krogh Building (BIO), and Danish Cancer Institute. Registration A letter of motivation and research interests is required. Course fee • Participant fee: 0 DKK • PhD student enrolled at SCIENCE: 0 DKK • PhD student from Danish PhD school Open market: 0 DKK • PhD student from Danish PhD school not Open market: 3000 DKK • PhD student from foreign university: 3000 DKK • Master's student from Danish university: 0 DKK • Master's student from foreign university: 3000 DKK • Non-PhD student employed at a university (e.g., postdocs): 3000 DKK • Non-PhD student not employed at a university (e.g., from a private company): 8400 DKK Cancellation policy • Cancellations made up to two weeks before the course starts are free of charge. • Cancellations made less than two weeks before the course starts will be charged a fee of DKK 3.000 • Participants with less than 80% attendance cannot pass the course and will be charged a fee of DKK 5.000 • No-show will result in a fee of DKK 5.000 • Participants who fail to hand in any mandatory exams or assignments cannot pass the course and will be charged a fee of DKK 5.000 Course fee and participant fee PhD courses offered at the Faculty of SCIENCE have course fees corresponding to different participant types. In addition to the course fee, there might also be a participant fee. If the course has a participant fee, this will apply to all participants regardless of participant type - and in addition to the course fee.

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