Sample Preparation and Separation Techniques in Bio-Analytical Chemistry
Provider: Faculty of Health and Medical Sciences

Activity no.: 3130-13-00-00There are no available seats 
Enrollment deadline: 09/08/2013
Date and time09.09.2013, at: 00:00 - 20.09.2013, at: 16:00
Regular seats16
Course fee10,680.00 kr.
LecturersSteen Honoré Hansen
ECTS credits6.30
Contact personJanne Steen Kjærulf    E-mail address: janne.kjaerulff@sund.ku.dk
Enrolment Handling/Course OrganiserPhD administration     E-mail address: phdkursus@sund.ku.dk
Aim and content
Aim
The course objectives are to illustrate the use of separation methods in drug analysis and bio-analysis. Topics will be separation mechanisms in gas chromatography, thin layer chromatography, high-performance liquid chromatography, super critical chromatography and capillary electrophoresis and the choice of separation technique for a given bio-analytical problem. Various techniques for sample preparation will be discussed in order to provide participants with sufficient knowledge to be able to develop bio-analytical methods efficiently.
The aim is that participants at the end of the course:
shall master a broad selection of modern separation and detection techniques as well as sample preparation techniques used for the analysis of biological samples.
shall know about analytical chemical scientific literature and relevant data bases.
shall be able to design methods for analysis of biological samples (e.g. urine and plasma) using advanced analytical chemical techniques.

Content
One half of the course will be devoted to the discussion of different separation techniques in chromatography and electrophoresis (gas chromatography, thin layer chromatography, high-performance liquid chromatography, super critical chromatography and capillary electrophoresis), and the advantages and limitations of each technique will be discussed in detail. The course will focus on separation mechanisms and on the right choice of separation technique for different bio-analytical problems as well as problems in drug analysis. The other half of the course will deal with sample preparation and with detection principles as these two parts of an analytical method depends on one another, thus being most important in the selection of the final method. Finally, method validation will be discussed.
Chromatographic theory: The partitioning process; the van Deemter plot; the capacity factor, resolution, efficiency and selectivity.
Separation mechanism: Stationary phase, mobile phase and buffer systems; column packing materials for chromatographic techniques; capillary technology for capillary electrophoresis; chiral separations.
Quantitative analysis, qualitative analysis and trace analysis.
Equipment: Mobile phase delivery; sample introduction systems; detection systems; data collection systems; automation.
Detection: Spectroscopic methods (UV, IR, NMR, MS, diode array, fluorescence); amperometric; radiochemical; derivatisation.
Sample preparation: Liquid-liquid extraction; solid phase extraction; dialysis; protein precipitation; on-line derivatisation; on-line sample preparation.
Method validation.

Participants
The course is relevant for all students performing or who is planning to performanalytical chemical analysis in the biosciences. A basic knowledge within chrmistry and physical chemistry as well as spectroscopy is an advantage.

Language
The course will normally be taught in English. However, if only Danish participants are enrolled the course will be taught in Danish.

Form
This course will be arranged once a year at The Danish University of Pharmaceutical Sciences. The course consists of about 25 hours of lectures/seminars and 20 hours of laboratory work. The course will run each day during two weeks. The first week lectures/seminars will take place from 9 to 12 o'clock in the morning. In the second week lectures will be held from 9 to 11 o'clock in the morning and laboratory work will take place 4 hours each afternoon.

The course will be concluded by a four hour written examination which will be assessed by the course director. All printed and written material as well as electronic calculators are allowed.

Teachers
Steen Honoré Hansen, Stig Petersen-Bjergaard; Nickolaj J. Petersen; Carsten Boye Knudsen.

Course location
Department of Pharmacy