PhD courses at University of Copenhagen

Molecular Pharmacology
Provider: Faculty of Health and Medical Sciences

Activity no.: 3116-14-00-00
	Enrollment deadline: 19/12/2013

Date and time	27.01.2014, at: 09:00 - 31.01.2014, at: 17:00
Regular seats	14
Course fee	9,240.00 kr.
Lecturers	Hans Bräuner
ECTS credits	5.00

Contact person	Marianne Wieslander Jørgensen E-mail address: marianne.joergensen@sund.ku.dk

Enrolment Handling/Course Organiser	PhD administration E-mail address: phdkursus@sund.ku.dk

Aim and content Learning objectives A student who has met the objectives of the course will be able to: a. Design and perform molecular pharmacological experiments using state-of-the-art methods b. Analyze pharmacological data using the program GraphPad Prism c. Present and discuss scientific articles in the field of molecular pharmacology Content Modern molecular pharmacology is characterized by the use of a broad variety of techniques in studies of receptor and transporter structure, function and pharmacology. In recent years increased focus has been on the use of fluorescent techniques to study signal transduction, activation mechanisms and trafficking. Another topic of current interest is the use of the increasing number of 3D structures and reliable models of receptors and transporters available to guide molecular pharmacological experiments, which have increased our understanding of the molecular basis for subtype selectivity, receptor activation and neurotransmitter transport. These topics will be covered during the course by lectures, practical exercises and student presentations of scientific articles on current molecular pharmacological topics. The lectures will cover: - State of the art molecular pharmacological assays - Theoretical background for the practical exercises The practical exercises will cover: - Pharmacological assays of G protein-coupled receptors and ligand-gated ion channels using: o measurement of intracellular Ca2+ levels by fluorescence probes o ?measurement of membrane potential by fluorescence probes o ?measurement of intracellular cAMP using time-resolved FRET and FRET-based biosensor o measurement of dynamic mass redestribution using label-free EPIC biosensor technique - Electrophysiological recordings in Xenopus oocytes using two-electrode voltage clamp - Quantitative evaluation of surface expression by ELISA and whole-cell binding - Use of GraphPad Prism software to evaluate pharmacological data Participants PhD students working in the field of pharmacology or with some experience in this field. Language English Form The course will be organised as an intensive 5-day course and will comprise approximately 26 hours of laboratory exercises, 10 lectures covering current topics in molecular pharmacology and student presentations of scientific articles. During the course it will be possible to discuss topics/problems in molecular pharmacology of special interest of the participants with the staff and fellow students. Evaluation: A written report of the performed experiments will have to be submitted two weeks after the course. During the course, participants will prepare a presentation of a scientific article. The presentation and the slides will be evaluated by the course leaders. Furthermore, participants should have been present and demonstrated a satisfactory level of activity during 90 per cent of the course. A grade of passed/not passed will be given. Experienced experimentalists from the Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen will supervise the lab-course. The participants will be supplied with study materials composed of notes and scientific publications. Course director Hans Bräuner-Osborne, professor, Department of Drug Design and Pharmacology, SUND, KU hbo@sund.ku.dk Teachers Professor Hans Bräuner-Osborne Associate professor Anders A. Jensen Associate professor Petrine Wellendorph All at Department of Drug Design and Pharmacology, SUND, KU Course secretary Marianne W. Jørgensen Dates January 27-31, 2014. Course location Molecular Pharmacology Group, University of Copenhagen, located at Symbion Research Park, Fruebjergvej 3, 2100 Copenhagen Ø.