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Human induced pluripotent stem cell culture, neural differentiation and gene-editing: in theory and in practice
Provider: Faculty of Health and Medical Sciences

Activity no.: 3630-16-00-01 
Enrollment deadline: 03/05/2016
Date and time30.05.2016, at: 09:00 - 03.06.2016, at: 16:00
Regular seats20
Course fee11,040.00 kr.
LecturersVanessa Hall
Kristine Freude
ECTS credits4.60
Contact personKaren Irene Brackley    E-mail address: karen.brackley@sund.ku.dk
Enrolment Handling/Course OrganiserPhD administration     E-mail address: phdkursus@sund.ku.dk

Aim and content
Learning objectives
A student who has met the objectives of the course will be able to:

Skills:
a. Freeze and thaw human induced pluripotent stem cells
b. Maintain and expand human induced pluripotent stem cells
c. Differentiate human induced pluripotent stem cells into divergent neural cell types (including astrocytes, oligodendrocytes and cortical neurons) using the latest 2D and 3D methodologies.
d. Analyze induced pluripotent stem cell-derived neural cells using different molecular methodologies.
e. Design CRISPRs for gene-editing of pluripotent stem cells

Knowledge:
f. Acquire a detailed understanding of the derivation of pluripotent stem cells
g. Acquire a detailed theoretical understanding of the states of pluripotency.
h. Acquire a detailed understanding of early neural developmental processes and molecular cues that govern neural patterning.
i. Gain detailed knowledge on state-of-the-art methods for culturing pluripotent stem cells.
j. Gain detailed knowledge on the latest methods for differentiating pluripotent stem cells into neural cell types (with focus on neurons, astrocytes and oligodendrocytes).
k. Gain expertise on use and application of CRISPRS in gene-editing of pluripotent stem cells.

Competences:
l. Will be able to develop custom-designed experiments in their own lab pertaining to pluripotent stem cell culture and differentiation into neural cells.
m. Will be able to custom-design CRISPRs for gene-editing of their own cell lines.


Content
The course follows the following topics:
Pre-implantation embryology, Pluripotent stem cell biology, Neural development and molecular patterning of the developing brain, Neural differentiation, Tissue culture of pluripotent stem cells, Tissue culture of different neural subtypes, CRISPR technologies and applications in pluripotent stem cells

Participants
PhD students, postdoctoral researchers and technicians embarking on culture of pluripotent stem cells with application for neural cell studies.

Language
English

Form
Lectures, group work, exercises, discussions, practical sessions in cell culture lab, poster presentations

Course directors
Vanessa Hall , Associate Professor, vh@sund.ku.dk
Karla Kristine Freude, Associate Professor, kkf@sund.ku.dk

Teachers
Lecturers:
Vanessa Hall , Associate Professor, University of Copenhagen, Denmark.
Kristine Freude, Associate Professor, University of Copenhagen, Denmark.
Yonglun Luo, Associate Professor, Århus University, Denmark, Denmark.
Benjamin Schmidt, Senior Researcher, Bioneer, Denmark.
Oskar Idås, Technical Sales Representative, Stem Cell Technologies, Sweden.

Keynote speakers:
Steven Alan Goldman, Professor, University of Rochester Medical Centre, U.S/University of Copenhagen, Denmark.
Laurent Roybon, Associate Professor, Lund University, Sweden
Andras Dinnyes, Professor, Biotalentum, Hungary.
Poul Hyttel, Professor, University of Copenhagen, Denmark.

Lab assistants:
Carlota Pires, PhD student, cp@sund.ku.dk
Yu Zhang, PhD student, yu.zhang@sund.ku.dk
Kai Xiong, PhD student, kai.xiong@sund.ku.dk
Tina Christoffersen, Technician, tch@sund.ku.dk

Course secretary
Vanessa Hall , Associate Professor, vh@sund.ku.dk

Dates
30th May 2016 – 3rd June 2016

Course location
Anatomy Building
Institute of Clinical Veterinary and Animal Sciences
Grønnegårdsvej 7, Frederiksberg C, DK 1870

Registration: Please register before 1st March 2015.

Admission to PhD students from Danish universities will be allocated on a first-come, first-served basis and according to the rules in force.
Applications from other participants will be considered after the last day of enrollment.

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