Sample Preparation and Separation Techniques in Bio-Analytical Chemistry
Provider: Faculty of Health and Medical Sciences

Activity no.: 3130-15-00-00 
Enrollment deadline: 07/08/2015
Date and time07.09.2015, at: 09:00 - 18.09.2015, at: 15:00
Regular seats18
Course fee10,560.00 kr.
ECTS credits6.30
Contact personJanne Kjærulf    E-mail address:
Enrolment Handling/Course OrganiserPhD administration     E-mail address:

Aim and content
Please note that dates are not yet decided.

Sample Preparation and Separation Techniques in Bio-Analytical Chemistry

The course objectives are to illustrate the use of separation methods in drug analysis and bio-analysis. Topics will be separation mechanisms in gas chromatography, high-performance liquid chromatography, super critical chromatography and capillary electrophoresis and the choice of separation technique for a given bio-analytical problem. Various techniques for sample preparation will be discussed in order to provide participants with sufficient knowledge to be able to develop bio-analytical methods efficiently.

Learning objectives
A student who has met the objectives of the course will be able to:

a. Develop and design methods for analysis of biological samples (e.g. urine and plasma) using advanced analytical chemical techniques
b. Shall master a broad selection of modern separation and detection techniques as well as sample preparation techniques used for the analysis of biological samples.
c. Shall know about analytical chemical scientific literature and relevant data bases
d. Practical experience with HPLC method development, ionpair chromatography, protein precipitation, GC-MS for analysis of biological samples, chiral capillary electrophoresis.

One half of the course will be devoted to the discussion of different separation techniques in chromatography and electrophoresis (gas chromatography, high-performance liquid chromatography, super critical chromatography and capillary electrophoresis), and the advantages and limitations of each technique will be discussed in detail. The course will focus on separation mechanisms and on the right choice of separation technique for different bio-analytical problems as well as problems in drug analysis. The other half of the course will deal with sample preparation and with detection principles as these two parts of an analytical method depends on one another, thus being most important in the selection of the final method. Finally, method validation will be discussed.
Chromatographic theory: The partitioning process; the van Deemter plot; the capacity factor, resolution, efficiency and selectivity.
Separation mechanism: Stationary phase, mobile phase and buffer systems; column packing materials for chromatographic techniques; capillary technology for capillary electrophoresis; chiral separations.
Quantitative analysis, qualitative analysis and trace analysis.
Equipment: Mobile phase delivery; sample introduction systems; detection systems; data collection systems; automation.
Detection: Spectroscopic methods (UV, IR, NMR, MS, diode array, fluorescence); amperometric; radiochemical; derivatisation.
Sample preparation: Liquid-liquid extraction; solid phase extraction; dialysis; protein precipitation; on-line derivatisation; on-line sample preparation.
Method validation.

The course is relevant for all students performing or who is planning to perform analytical chemical analysis in the biosciences. A basic knowledge within chemistry and physical chemistry as well as spectroscopy is an advantage.

The course will normally be taught in English. However, if only Danish participants are enrolled the course will be taught in Danish.

The course consists of about 25 hours of lectures/seminars and 20 hours of laboratory work. The course will run each day during two weeks. The first week lectures/seminars will take place from 9 to 12 o'clock in the morning. In the second week lectures will be held from 9 to 11 o'clock in the morning and laboratory work will take place 4 hours each afternoon.

The course will be concluded by a four hour written examination, which will be assessed by the course director. All printed and written material as well as electronic calculators are allowed.

Course director
Nickolaj J. Petersen, Associate Professor, Department of Pharmacy, University of Copenhagen.

Stig Pedersen-Bjergaard, Professor, Department of Pharmacy
Jörg Kutter, Professor, Department of Pharmacy
Kasper Dyrberg Rand, Associate Professor, Department of Pharmacy

Course secretary
Janne Kjærulff,

September/October 2015

Course location
Department of Pharmacy, Universitetsparken 2, 2100 Copenhagen.

Registration: Please register before 7 August 2015

Admission to PhD students from Danish universities will be allocated on a first-come, first-served basis and according to the rules in force.
Applications from other participants will be considered after the last day of enrollment.