Login for PhD students at UCPH      Login for others
Human induced pluripotent stem cell culture, neural differentiation and gene-editing: in theory and in practice
Provider: Faculty of Health and Medical Sciences

Activity no.: 3630-17-00-00 
Enrollment deadline: 01/03/2017
Date and time29.05.2017, at: 09:00 - 02.06.2017, at: 18:00
Regular seats15
Course fee10,560.00 kr.
LecturersVanessa Hall
Kristine Freude
ECTS credits4.60
Contact personBetina Wingreen Jensen    E-mail address: bwj@sund.ku.dk
Enrolment Handling/Course OrganiserPhD administration     E-mail address: phdkursus@sund.ku.dk

Aim and content
This course is free of charge for PhD students at Danish universities (except Copenhagen Business School). Special rules apply for research year students enrolled at Faculty of Health and Medical Sciences at UCPH. All other participants must pay the course fee.
Anyone can apply for the course, but if you are not a PhD student, you will be placed on the waiting list for the course until enrollment deadline. After the deadline of enrollment, available seats will be allocated to students on the waiting list.

Learning objectives
A student who has met the objectives of the course will be able to:

Skills:
a. Freeze and thaw human induced pluripotent stem cells
b. Maintain and expand human induced pluripotent stem cells
c. Differentiate human induced pluripotent stem cells into divergent neural cell types (including astrocytes, oligodendrocytes and cortical neurons) using the latest 2D and 3D methodologies
d. Analyze induced pluripotent stem cell-derived neural cells using different molecular methodologies
e. Design CRISPRs for gene-editing of pluripotent stem cells

Knowledge:
f. Acquire a detailed understanding of the derivation of pluripotent stem cells
g. Acquire a detailed theoretical understanding of the states of pluripotency
h. Acquire a detailed understanding of early neural developmental processes and molecular cues that govern neural patterning
i. Gain detailed knowledge on state-of-the-art methods for culturing pluripotent stem cells
j. Gain detailed knowledge on the latest methods for differentiating pluripotent stem cells into neural cell types (with focus on neurons, astrocytes and oligodendrocytes)
k. Gain expertise on use and application of CRISPRS in gene-editing of pluripotent stem cells

Competences:
l. Will be able to develop custom-designed experiments in their own lab pertaining to pluripotent stem cell culture and differentiation into neural cells
m. Will be able to custom-design CRISPRs for gene-editing of their own cell lines

Content
The course follows the following topics:
Pre-implantation embryology, Pluripotent stem cell biology, Neural development and molecular patterning of the developing brain, Neural differentiation, Tissue culture of pluripotent stem cells, Tissue culture of different neural subtypes, CRISPR technologies and applications in pluripotent stem cells

Participants
PhD students, postdoctoral researchers and technicians embarking on culture of pluripotent stem cells with application for neural cell studies.


Relevance to graduate programmes
The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH:

All graduate programmes


Language
English

Form
Lectures, Keynote lectures, Group exercises, Practical laboratory work, Poster presentations


Course director
Kristine Freude, Associate Professor, Dept. of Veterinary Clinical and Animal Sciences, kkf@sund.ku.dk
Vanessa Hall, Associate Professor, Dept. of Veterinary Clinical and Animal Sciences, vh@sund.ku.dk

Teachers
Lecturers:
Vanessa Hall, Associate Professor, University of Copenhagen
Kristine Freude, Associate Professor, University of Copenhagen
Yonglun Luo, Associate Professor, Århus University, Denmark, Denmark
Benjamin Schmid, Senior Researcher, Bioneer, Denmark
Oskar Idås, Technical Sales Representative, Stem Cell Technologies, Sweden

Keynote Speakers:
Morten Meyer, Associate Professor, University of Southern Denmark
Oliver Brüstle, Professor, University of Bonn
Poul Hyttel, Professor, University of Copenhagen

Lab Assistants:
PhD Students, PostDocs and Technicians from the department


Dates
29th May – 2nd June 2017

Course location
University of Copenhagen
Dept. of Veterinary Clinical and Animal Sciences
Grønnegårdsvej 7
1870 Frederiksberg C

Registration
Please register before 1st March 2017

The course is limited to a maximum of 20 participants.
Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules.
Applications from other participants will be considered after the last day of enrolment.

Search
Click the search button to search Courses.


Course calendar
See which courses you can attend and when
JanFebMarApr
MayJunJulAug
SepOctNovDec



New courses
Courses are published regularly. High demand courses are announced in spring and autumn.


Learn which courses are announced on fixed dates