Graduate School of health and Medical Sciences

Phage Display; Screening of proteins with DNA encoded peptide libraries to find new types of biopharmaceutical lead structures
Provider: Faculty of Health and Medical Sciences

Activity no.: 3139-17-00-00
	Enrollment deadline: 21/05/2017

Date and time	12.06.2017, at: 09:00 - 23.06.2017, at: 17:00
Regular seats	12
Course fee	17,040.00 kr.
Lecturers	Brian Lohse
ECTS credits	7.20


Enrolment Handling/Course Organiser	PhD administration E-mail address: phdkursus@sund.ku.dk

Aim and content This course is free of charge for PhD students at Danish universities (except Copenhagen Business School). Special rules apply for research year students enrolled at Faculty of Health and Medical Sciences at UCPH. All other participants must pay the course fee. Anyone can apply for the course, but if you are not a PhD student, you will be placed on the waiting list for the course until enrollment deadline. After the deadline of enrollment, available seats will be allocated to students on the waiting list. Course aim This course aims primarily towards that the PhD student achieves practical experience and theoretical knowledge of the Phage Display technology, covering some of the most used methods in molecular biology, e.g. virus infection of bacteria, DNA-encoding for peptide libraries and ELISA binding assays. On the experimental and practical level it is the aim of the course the students acquire the knowledge of methods that will enable them to do screening of different protein targets using large peptide libraries using important methods, which are fundamental in pharmacology and medicinal chemistry today. These techniques will demonstrate to the students how powerful molecular biology are to facilitate identification of new peptide lead structures in 10 days, which would take years if using conventional techniques e.g. synthetic small molecule chemistry. The participants will learn how to handle and grow bacteria, infecting them with virus and isolating hits. They will learn how to precipitate the phages and isolate their DNA, and sequence it. The PhD students will also gain insight into the mechanisms by which virus can infect bacteria and how this infection pathway can be taken advantage of and turned into a powerful tool, to aid the researcher in identifying new lead structures that can be used in the pursuit of new pharmaceuticals. The PhD student will gain a good understanding of how binding motifs and interactions, identified in the phage display can be elucidated, using techniques like Biacore and H/D-Exchange. Furthermore, the students are allowed to bring their own research project targets, which increase the value of such a course in the sense that the students have the possibility to gain new lead structures for their own projects. Problem Based and Research Based Learning will be the fundament throughout the course. These methods and phage display is cutting edge technology and used on a daily basis in the medicinal industry and in molecular drug research as an essential step towards obtaining new lead structures that can be developed into new potent biopharmaceuticals. Learning objectives A student who has met the objectives of the course will be able to: 1. Use DNA-encoded libraries 2. Screen a specific protein target 3. Identify a potential binder and/or inhibitor Content The course covers the following subjects: Molecular Biology: • Recombinant DNA technique • DNA sequencing • Cell growth • Recombinant expression by transfection in host cells • ELISA Medicinal Chemistry • Peptide chemistry • Combinatorial peptide libraries • Fluorescense-based functional assays • Binding/ inhibition studies The instruction and lab. manual is written by the teacher (primary) responsible for the course and as a supplement relevant articles from the literature will be added. Describe the course curriculum in terms of scientific topics covered. Participants The PhD students are expected to have acquired the following competences and skills, before they start the course corresponding to the following courses/ modules: Biochemistry/ Bioorganic chemistry and/ or microbiology and lab experience within one of these fields. The course is open for all SUND PhD students and researcher and lab. technicians from industry and PhD students from other universities will also be accepted. Describe the target group of the course and required qualifications (if any) by the participating PhD students. Relevance to graduate programmes The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH: All graduate programmes Select either “all graduate programmes” or up to three relevant graduate programmes. If the course is organised by a graduate programme, please include this in your selection. See information about graduate programmes. Language If all attendants speak Danish, then in Danish, otherwise in English. Form The course will be held outside the block structure, in order not to conflict with obligatory lab. courses. The students will be accessed based on active participation, constituting 90 %. More than 10 % absence will exclude the student from taking the written exam. The final evaluation is based upon an accepted report and a multiple choice test lasting 2 hours, which are equally weighted. Practical laboratory exercises alternating with review and discussion of underlying theory. Students work in teams of two. For instance: Lectures, group work, discussions, poster presentations, exercises or others. Assessment The course is accessed based on 3 important elements: 1) Active participation in the lab. ( min. 90 %) 2) Approved report. 3) Multiple choice test (min. 50 % correct). Passed/ not-passed. The written test is a multiple choice test, consisting of 33 questions with 4 possibilities in each. All questions are weighted equally and the test lasts 2 hours. The approved report from each student will be graded and make up for 50 % of the final grade. The grade from the multiple choice test will make up for the rest of the final grade passed/ not passed. Course director Brian Lohse, PhD, Associate Professor, Chemical Biology & Molecular Biology, and CSO of EpiDiscoverY University of Copenhagen, Faculty of Health and Medical Sciences, Department of Drug Design and Pharmacology, Jagtvej 160, 3rd floor, office C.327, 2100 Copenhagen Ø. Office Phone: (+45) 35336658, Mobile Phone: (+45) 93565431 E-mail: bril@sund.ku.dk Group Homepage: http://drug.ku.dk/research/molecular_and_cellular_pharmacology/epidiscovery-group Teachers Brian Lohse and Lab. Technician Janet Kasule, Department of Drug Design and Pharmacology. Invited Guest Teachers (1 hour lecture each): Novozymes A/S (PhD, Torben V. Borchert), Dako A/S (PhD, Peter D. Skottrup) Gentofte Hospital (MD, Pal B. Szecsi) Dates 12-23 June 2017 Course location Department of Drug Design and Pharmacology, ILF, Building 22. Registration Please register before 1 May 2017 Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules. Applications from other participants will be considered after the last day of enrolment. Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.

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