Graduate School of health and Medical Sciences

Light Microscopy and Confocal
Provider: Faculty of Health and Medical Sciences

Activity no.: 3400-17-00-01
	Enrollment deadline: 01/07/2017

Date and time	14.08.2017, at: 09:00 - 01.09.2017, at: 16:30
Regular seats	20
Course fee	5,520.00 kr.
Lecturers	Clara Prats
ECTS credits	8.00

Contact person	Ida Marie Bergman Rasmussen E-mail address: ida.mbr@sund.ku.dk

Enrolment Handling/Course Organiser	PhD administration E-mail address: phdkursus@sund.ku.dk

Aim and content This course is free of charge for PhD students at Danish universities (except Copenhagen Business School). Special rules apply for research year students enrolled at Faculty of Health and Medical Sciences at UCPH. All other participants must pay the course fee. Anyone can apply for the course, but if you are not a PhD student, you will be placed on the waiting list for the course until enrollment deadline. After the deadline of enrollment, available seats will be allocated to students on the waiting list. Course title Light Microscopy Learning objectives A student who has met the objectives of the course will be able to: 1. Set up the light path of a light microscope properly to avoid artifacts (set Köhler illumination) 2. Understand the properties of light and the main contrast techniques used in light microscopy (BF, Phase contrast, Drak field, DIC and polarized light) 3. Understand the principles of fluorescence and fluorescence microscopy 4. Set up an imaging profile with a confocal microscope (LSM) to avoid detection artifacts Please describe the students’ learning objectives in terms of; knowledge, skills and/or competences. Depending on the course curriculum, the distribution between the learning objectives may vary. Describe the obtained learning objectives with words such as: ability to identify, explain, assess, define, describe, have insight, understand, demonstrate, analyse, conclude, compare, diagnose, theorise, put into perspective etc. Please state 3-5 learning objectives. Content This course in Light Microscopy follows the series of successful courses in Light Microscopy, Confocal and Fluorescence Microscopy and Electron Microscopy. This course consists of two modules and is suitable not only for beginners in microscopy, but also for those who already use the microscope in their work and now want to extend their knowledge of basic principles and more specialised techniques. The course is run in collaboration with The Royal Microscopical Society. Module 1 (first week) provides an essential grounding in the basic principles of microscopy, including: the limitations of the eye; resolution, contrast and magnification; refraction; lenses and images; conjugate planes; methods of illumination; diffraction, aperture and resolution; generation of contrast; introduction to bright-field, dark ground, phase contrast, polarised light, differential interference contrast and fluorescence; principles of image recording. The module also covers the basic principles of electron microscopy. Module 2 (second week) covers the characteristics and alignment of the fluorescence and confocal microscopes; the selection of fluorophores and corresponding filter sets; immunofluorescent and fluorescent affinity staining of biological specimens. Consideration will also be given to the use of cooled-CCD cameras. The principles of FRET and FLAP will be covered and demonstrated in practice. In addition, methods for producing images representing two- and three-dimensional data sets from computer-based data sets and deconvolution techniques will be described and demonstrated in practice. CFIM (www.cfim.ku.dk) will furnish state-of-the-art fluorescence and personal confocal microscopes to give students an opportunity to get hands-on experience. Participants PhD students who use light microscopes Language English Form Lectures, exercises Course director Clara Pratts, cprats@sund.ku.dk Teachers Klaus Qvortrup, Clara Prats, Daniel Zicha, Laure Plantard, Peter Evennett, Andrew Scott, Thomas Braunstein Dates 14-18 Aug + 28 Aug - 1 Sep. Course location Panum Institute Registration Please register before 1 July 2017 Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.

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