Graduate School of health and Medical Sciences

Single cell transcriptomics
Provider: Faculty of Health and Medical Sciences

Activity no.: 3665-18-00-00	There are no available seats
	Enrollment deadline: 01/08/2018

Date and time	27.09.2018, at: 09:00 - 03.10.2018, at: 17:00
Regular seats	15
Course fee	13,680.00 kr.
Lecturers	Konstantin Khodosevich
ECTS credits	5.70

Contact person	MoMed Team E-mail address: momed@bric.ku.dk

Enrolment Handling/Course Organiser	PhD administration E-mail address: phdkursus@sund.ku.dk

Aim and content This course is free of charge for PhD students at Danish universities (except Copenhagen Business School). All other participants must pay the course fee. Anyone can apply for the course, but if you are not a PhD student, you will be placed on the waiting list for the course until enrollment deadline. After the deadline of enrollment, available seats will be allocated to students on the waiting list. Course title Single cell transcriptomics Learning objectives A student who has met the objectives of the course will be able to: 1. Wet-lab: Single cell analysis by SMARTSeq2 2. Wet-lab: Single nucleus analysis by SMARTSeq2 3. Wet-lab: Single cell analysis by 10x Chromium 4. Dry lab: To understand how single cell transcriptomics data can be clustered by cell type 5. Dry lab: To understand how cells can be placed along their developmental trajectories 6. Dry lab: To understand how genes can be grouped into co-expressed modules Content Single cell transcriptomics using plate-based method, SMARTSeq2, and droplet-based method, 10x Chromium. Whole cell and nucleus transcriptomics Processing of single cell data using Seurat and Pagoda (for the data processing part, the participants will be subdivided into unexperienced and experienced users) Participants are expected to present their projects in form of oral presentation Participants PhD students at any year of their studies, some experience in molecular biology (molecular cloning, PCR, work with RNA) is required. There is no prerequisite knowledge of programming or data analysis tools, and both experienced sequencing data analysis users as well as unexperienced ones can attend the course. However, for the data analysis part, the participants will be subdivided into 2 groups and the training will be provided at beginners and advanced levels. If you are enrolled, please send letter of motivation for attending the course before registration deadline to: momed@bric.ku.dk Relevance to graduate programmes The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH: All graduate programmes Language English Form Wet-lab course, lectures, group work, exercises and discussions. Expect an 8-9 hour working day, also during the weekend. Course director Konstantin Khodosevich, Associate professor, BRIC, konstantin.khodosevich@bric.ku.dk Tune H Pers, Associate professor, CBMR, tune.pers@sund.ku.dk Peter Kharchenko, Harvard Medical School, Peter_Kharchenko@hms.harvard.edu Teachers Konstantin Khodosevich, Dr, BRIC Ulrich Pfisterer, Dr, BRIC Samuel Demharter, Dr, BRIC Tune H Pers, Dr, CBMR Julie Lee, Dr, CBMR Pascal Timshel, Phd student, CBMR Dylan Rausch, Phd student, CBMR Peter Kharchenko, Dr, Harvard Medical School Dates 27.09.18-03.10.18 Course location BRIC Registration Please register before 1.08.18. If you are enrolled, please send a letter of motivation for attending the course before registration deadline to: momed@bric.ku.dk Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules. Applications from other participants will be considered after the last day of enrolment. Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.

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