Graduate School of health and Medical Sciences

Genome editing using CRISPR/Cas9
Provider: Faculty of Health and Medical Sciences

Activity no.: 3429-18-00-00
	Enrollment deadline: 01/09/2018

Date and time	04.12.2018, at: 09:00 - 07.12.2018, at: 16:00
Regular seats	25
Course fee	4,920.00 kr.
Lecturers	Morten Frödin
ECTS credits	2.80

Contact person	MoMed Team E-mail address: momed@bric.ku.dk

Enrolment Handling/Course Organiser	PhD administration E-mail address: phdkursus@sund.ku.dk

Aim and content This course is free of charge for PhD students at Danish universities (except Copenhagen Business School). All other participants must pay the course fee. Anyone can apply for the course, but if you are not a PhD student, you will be placed on the waiting list for the course until enrollment deadline. After the deadline of enrollment, available seats will be allocated to students on the waiting list. Learning objectives A student who has met the objectives of the course will be able to: 1. Have obtained a basic knowledge of the underlying principles, mechanism of action and the potential as well as the limitations of genome editing using CRISPR/Cas9. 2. Be able to design target specific CRISPR/Cas9 reagents and incorporate their use in own research project. 3. Be able to analyse and assess own results as well as those obtained by others using CRISPR/Cas9 and be prepared for the upcoming developments in the field of genome editing. Content This theoretical course will provide an overview of the principles and use of the CRISPR/Cas9 system for genome editing, from the basics to the most advanced applications. Participants will learn how to design CRISPR/Cas9 reagents for a desired genomic target, select appropriate delivery vectors and editing strategy for a given project, principles for generation of gene knockout or knockin-modified/base-edited cells and become familiar with the various methods for mutation detection using the current technologies. Genome-wide CRISPR screening, CRISPRi, CRISPR paint and other CRISPR applications will be introduced. Advanced research stories using CRISPR/Cas9 will be presented. The course includes the one-day mini-symposium on “Precise Genome Editing using CRISPR/Cas9” featuring international speakers form leading laboratories Design of CRISPR/Cas9 reagents (gRNAs) using online tools; delivery methods (transfection/transduction) and associated vector systems; basic rules for knockout and knockin, including design of knockin targeting constructs; enrichment strategies for genome edited cells (FACS, antibiotics selection); methods for detection of CRSPR/Cas9-induced knockout/knockin/base editing mutation and clone validation; off-target considerations and rescue strategies; Alternative genome editing tools (TALENs/ZFNs) and CRISPR applications (genome-wide screening/CRISPRi/CRISPRa/CRISPR paint etc), animal engineering; examples of research applications of CRISPR/Cas9 technology. Participants The course is intended for PhD students who have a desire to use or are already applying CRISPR/Cas9-based genome editing in their research. No previous experience in genome editing is required. The course has room for 25 participants. Relevance to graduate programmes The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH: All graduate programmes Language English Form Lectures, troubleshooting workshops Course director Morten Frödin, associate professor, BRIC, University of Copenhagen, morten.frodin@bric.ku.dk Eric P. Bennett, associate professor, Copenhagen Center for Glycomics, University of Copenhagen, epb@sund.ku.dk Cord H. Brakebusch, professor, BRIC, University of Copenhagen, cord.brakebusch@bric.ku.dk Teachers National and international expert users of CRISPR/Cas9 Greg Davis, MilliporeSigma, USA Jason Potter, ThermoFisher, USA Aliaksandra Radzisheuskaya, BRIC Karl Agger, BRIC Krister Wennerberg, BRIC Rasmus Bak, University of Aarhus Petr Kasparek, Czech Centre for Phenogenomics David R. Liu, Harvard, USA Katie Pawelcsak, NERx Biosciences Shengdar Q. Tsai, St Jude Children´s Research Hospital, Memphis, USA Wen Xue, University of Massachusetts Medical School, USA Jacob Corn, Berkeley, USA Massimo Squatrito, Spanish National Cancer Research Centre (CNIO) Dates December 4-7, 2018 + Includes Mini-symposium “Genome Editing using CRISPR/Cas9”, December 7, 2018 Course location Biotech Research & Innovation Centre (BRIC), Ole Maaløes vej 5, 2200 København N and the Panum Institute, Blegdamsvej 3, 2200 København N. Registration Please register before September 1st 2018 All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.

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