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Experimental Animal Nutrition and Physiology (only for PhD students)
Provider: Faculty of Health and Medical Sciences
Activity no.: 3167-18-00-00
Enrollment deadline: 01/11/2018
Date and time
20.11.2018, at: 08:00 - 24.01.2019, at: 16:00
Regular seats
12
Course fee
4,000.00 kr.
Lecturers
Mette Olaf Nielsen
ECTS credits
9.00
Enrolment Handling/Course Organiser
PhD administration E-mail address: phdkursus@sund.ku.dk
Aim and content
This course is free of charge for PhD students at Danish universities (except Copenhagen Business School). There may be a fee for other participants, please contact the course director.
Only PhD students can apply for the course.If you are not a PhD student please contact the course director.
Learning objectives
A student who has met the objectives of the course will be able to:
Knowledge
• describe the basic principles behind experimental methods for measuring nutritional characteristics of feedstuffs, feed intake, chewing activity, in vivo and in vitro digestibility,
• describe the methods for measuring digestibility in different segments of the digestive tract in monogastric and ruminant animals by use of cannulation and marker techniques, passage rate and digestion kinetics
• describe the theoretical background of energy transfer from cellular to the whole body level, techniques and methods for measuring nitrogen, energy balances, heat production and substrate oxidation
• describe the use of molecular biological techniques in analysis of cell and tissue function
• describe catheter techniques used to study tissue and organ nutrient fluxes, practical guidelines for sampling of blood, rumen fluid etc,
• describe appropriate experimental designs and basic statistical approaches for use with these methodologies
• describe the legislation, handling and management of laboratory animals
• integrate the outcomes from the methodologies, experimental design work and legislative aspects to obtain an understanding of the ethical use of animals in research
Skills
• be able to describe principles and methods used in selected in vivo demonstrations
• be able to evaluate experimental results/data from different in vivo measurements
• be able to discuss limitations, advantages and their potential applications of the different experimental methods
• be able to interpret experimental results based on theoretical knowledge
• be able to design experimental animal protocols and use relevant legislation for conducting animal experiments in an ethical way
• be able to prepare a journal article style report
Competences
• ability to cooperate with other researchers, both inter and intra disciplinary at different levels and with different degree of responsibility
• ability to adopt above described knowledge and skills
• ability to disseminate methodology and obtained results to professionals, lay persons and general public
• ability to perform animal experiments according to Council of Europe 1993 resolution category B
• ability to present results of research according to academic principles
Content
1) Presentations during 6 different week modules of experimental techniques used for assessment of whole animal function and metabolism of nutrients at the whole animal and organ level (in vivo/in vitro) with a focus of larger (farm) animal species. This covers both a theoretical introduction as well as hands-on demonstrations of key techniques used for studies relating to:
a. Digestibility and feed analyses: digestibility trials, digestibility markers, theory of sampling
b. Whole body energy and protein metabolism: calorimetric and stable isotope methods
c. Feed intake and chewing: rumen sampling, chewing pattern determination, in sacco techniques
d. In situ determination of organ function, metabolic and endocrine adaptability: catheterization and sampling techniques, in vivo pharmacological tolerance tests
e. In vitro digestibility and in vitro fermentation techniques: in vitro gas production, inocculants, gas analyses, mathematical description of gas production curves (rate of fermentation)
2) A 1 week module (30 h) equivalent to the educational requirements by Council of Europe (category b) and Denmark for persons working with experimental animals or assisting in animal experiments. This covers This covers both a theoretical introduction as well as hands-on demonstrations of key techniques used for studies relying on use of laboratory animals with hands-on practicals regarding laboratory animal handling and minor procedures in rats and mice
3) Principles necessary for planning and implementing in vivo or in vitro scientific investigations and for critical evaluation of results obtained from investigations employing the demonstrated methods. This includes such aspects as protocol planning, experimental design, data evaluation and knowledge of advantages and limitations of the methods demonstrated relating to alternative experimental methods.
4) Preparation of a journal article style report based on one of the 6 week modules mentioned in 1) according to the students own choice.
Participants
The course is co-taught with the course Experimental Animal nutrition and Physiology for Animal Science MSc students. During the practical demonstrations and presentations at the weekly seminars, the PhD student will be working in a group with MSc students. However, the PhD students will be examined separately corresponding to what can be expected at this level of education.
PhD students are expected to have a basic knowledge about animal (or human) nutrition.
Relevance to graduate programmes
The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH:
Veterinary and Animal Sciences
Herd and Population-oriented Research (HERD)
Public Health and Epidemiology
Language
English
Form
In this course, introduction to experimental techniques and their strengths and weaknesses are introduced in theoretical lectures (4 hours per week module), and students will thereafter obtain hands-on experience with selected techniques at weekly practical exercises, involving live experimental animals and laboratory exercises using animal-derived biological material (8 hours per week module). Learning outcomes will be presented by students at weekly seminars. Individual activities include reviewing and presentation of handed-out scientific papers as well as seminar discussions of the presented experimental techniques. Particular emphasis will be given on the ability of the PhD student to evaluate experimental designs, employed experimental techniques and approaches in relation to aims, obtained results and the conclusions which can be drawn from the obtained results, and critical discussion of other relevant experimental techniques.
Course director
Mette Olaf Nielsen, Professor MSO, Department of Veterinary and Animal Sciences, mette.olaf.nielsen@sund.ku.dk
Course location
Department of Veterinary and Animal Sciences
Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.
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