Graduate School of health and Medical Sciences

Hands-on course in TEM & SEM of mammalian cells
Provider: Faculty of Health and Medical Sciences

Activity no.: 3251-19-00-00
	Enrollment deadline: 25/10/2019

Date and time	25.11.2019, at: 08:00 - 04.12.2019, at: 15:00
Regular seats	12
Course fee	16,680.00 kr.
Lecturers	Poul Hyttel
ECTS credits	6.20

Contact person	Betina Wingreen Jensen E-mail address: bwj@sund.ku.dk

Enrolment Handling/Course Organiser	PhD administration E-mail address: phdkursus@sund.ku.dk

Aim and content This course is free of charge for PhD students at Danish universities (except Copenhagen Business School), and for PhD students at graduate schools in the other Nordic countries. All other participants must pay the course fee. Anyone can apply for the course, but if you are not a PhD student, you will be placed on the waiting list for the course until enrollment deadline. After the deadline of enrollment, available seats will be allocated to students on the waiting list. Learning objectives A student who has met the objectives of the course will be able to: 1. Plan relevant ultrastructural studies according to cell types and cell biological aspects in question 2. Fix and dehydrate cells and process them further for embedding and sectioning for transmission electron microscope and for critical point drying and sputter coating for scanning electron microscopy 3. Acquire micrographs using transmission and scanning electron microscopes 4. Perform initial ultrastructural evaluation of the acquired images 5. Theoretically evaluate the potentials of further processing for ultrastructural immunocytochemistry, quantification and other more advanced ultrastructural methodologies Content Transmission (TEM) and scanning electron microscopy (SEM) are specialized technologies that are often used when cell biological aspects need to be visualized at the maximum resolution. In their conventional form, TEM focuses on cell visualization in ultra-thin two-dimensional sections giving access to all internal cell components represented in a section, whereas SEM focuses on a three-dimensional representation of surface structures of cells. The two visualization principle demands different specimen preparations. This hands-on PhD course allows the students to get insight to and train fixation (including primary and secondary fixation), dehydration, embedding for TEM, semi-thin sectioning and staining for light microscopy, ultra-thin sectioning and contrasting for TEM, critical point drying and sputter coating for SEM, acquisition of TEM and SEM micrographs as well as qualitative and quantitative analyses of the results. Preparation of specimens from three-dimensional tissue blocks as well as from two-dimensional cell cultures will be addressed. Hands-on exercises will, furthermore, address quantification of ultrastructural data. Finally, the students will acquire theoretical knowledge about more advanced applications in electron microscopy including immunocytochemistry and alternative visualization methodologies. Particular focus will be given to the use of TEM and SEM for detection of cellular disease phenotypes in neurons and glia cells differentiated from patient-specific induced pluripotent stem cells (iPSCs) serving as neural in vitro cell models for neurodegeneration. Participants The course is designed for PhD students with a background in medicine, veterinary medicine, cell and molecular biology, human biology and biology/biotechnology. Participation in the course requires general knowledge in cell biology and histology. Due to the hands-on training a maximum of 12 students can be enrolled. Relevance to graduate programmes The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH: Medical and Molecular Imaging Neuroscience Veterinary and Animal Sciences Language English. Form Lectures, exercises and discussions. Course director Poul Hyttel, Professor, Department of Veterinary and Animal Sciences, University of Copenhagen, poh@sund.ku.dk Teachers Poul Hyttel, Professor, Department of Veterinary and Animal Sciences, University of Copenhagen Gert H Hansen, Associate Professor, RNA and Gene Medicine Program, University of Copenhagen Klaus Qvortrup, Professor, Department of Biomedical Sciences, University of Copenhagen Jens Randel Nyengaard, Professor, Department of Clinical Medicine Aarhus University Michael Hansen, Cell Biologist, Department of Plant and Environmental Sciences, University of Copenhagen Maria Pihl, Laboratory Technician, Department of Veterinary and Animal Sciences, University of Copenhagen Dates 25 November to 4 December 2019. Course location Department of Veterinary and Animal Sciences, University of Copenhagen, Groennegaardsvej 7, 1870 Frederiksberg C Registration Please register before 25 October 2019. Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules. Applications from other participants will be considered after the last day of enrolment. Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.

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