Graduate School of health and Medical Sciences

Mass Spectrometry Coupled to Separation Techniques in Bioanalytical Chemistry
Provider: Faculty of Health and Medical Sciences

Activity no.: 3132-21-00-00
	Enrollment deadline: 10/12/2020

Date and time	11.01.2021, at: 09:00 - 15.01.2021, at: 16:00
Regular seats	20
Course fee	6,240.00 kr.
Lecturers	Christian Janfelt
ECTS credits	4.10

Contact person	Marianne Wieslander Jørgensen E-mail address: marianne.joergensen@sund.ku.dk

Enrolment Handling/Course Organiser	PhD administration E-mail address: phdkursus@sund.ku.dk

Aim and content This course is free of charge for PhD students at Danish universities (except Copenhagen Business School), and for PhD students at graduate schools in the other Nordic countries. All other participants must pay the course fee. Anyone can apply for the course, but if you are not a PhD student at a Danish university, you will be placed on the waiting list until enrollment deadline. This also applies to PhD students from Nordic countries. After the enrollment deadline, available seats will be allocated to applicants on the waiting list. Learning objectives A student who has met the objectives of the course will be able to: Knowledge •explain the principles of different ionization techniques and mass analyzers, their areas of application and possible limitations •understand the most important concept required for reading literature on mass spectrometry •understand the use of mass spectrometry in a range of related areas such as pharmaceutical sciences, forensics and protein research Skills •Plan qualitative and quantitative experiments with mass spectrometry in a range of scientific fields •Critically interpret data obtained using a range of techniques based on mass spectrometry •Use mass spectrometry for structure elucidation and quantitation Competences •Select the appropriate ionization technique and mass spectrometer for a given scientific problem •Communicate to people within and outside the field of mass spectrometry on the use of mass spectrometry in different fields of research Content The course will introduce the participant to the basic concepts of mass spectrometry. The course will present important principles and practice of LC-MS, LC-MS/MS and GC-MS, and during the course the following issues will be presented and discussed: •Introduction to basic concepts of mass spectrometry (MS). •Introduction to tandem mass spectrometry (MS/MS). Ionisation techniques: electrospray ionisation, atmospheric pressure chemical ionisation, atmospheric pressure photo ionization, electron and chemical ionization, and matrix-assisted laser desorption ionization. •Mass analyzers (quadrupoles - single/triple, ion trap, TOF, Orbitrap, FT-ICR and sector-field). •Quantitative analysis using MS (MS/MS). •Structure elucidation by means of MS (accurate mass, MS/MS and MSn). •Chromatographic aspects regarding MS. •Development of LC-MS methods. •Application of MS in drug metabolism, peptide- and protein analysis, pharmacokinetics and other related disciplines. •New trends in mass spectrometry, including mass spectrometry imaging and ambient ionization techniques. Participants The course is relevant for people performing or planning to perform chemical analysis with high sensitivity and selectivity using separation techniques coupled to mass spectrometry detectors. A basic knowledge in separation science covering the techniques GC, LC and CE is an advantage. Relevance to graduate programmes The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH: All graduate programmes Language English Form The 5-day course will comprise approximately 30 lectures given by experienced experts in mass spectrometry from academia and industry. The participants will be involved in the teaching-programme by working in groups on various problems related to the lectures. The group exercises will focus on formulating strategies and solutions for analytical problems involving topics of the course. At the beginning of the course, each student will receive some problems, which she/he has to solve during the course or within two weeks after the course. Each student is expected to write a short report (4-5 pages) presenting the solution of the problems. This report must be delivered to the course director within two weeks after the last day of the course. The report is assessed as "passed" or "not passed". To obtain the course certificate the student must obtain the assessment “passed” for the report. Course director Associate professor Christian Janfelt, Department of Pharmacy, The Faculty of Health and Medical Sciences, University of Copenhagen, (christian.janfelt@sund.ku.dk) Teachers Christian Janfelt, Kasper Rand, Andreas Kretschmann, Lars Ynddal, Line Rørbæk Olsen a.o. Dates January 11th to 15th 2021. Course location TBA, Universitetsparken 2, DK-2100 Copenhagen. Registration Please register before December 1st 2020 Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules. Applications from other participants will be considered after the last day of enrolment. Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.

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