Graduate School of health and Medical Sciences

Induced Pluripotent Stem Cells, Gene-editing and Brain Cell Model
Provider: Faculty of Health and Medical Sciences

Activity no.: 3630-22-00-00
	Enrollment deadline: 06/04/2022

Date and time	06.05.2022, at: 00:00 - 12.05.2022, at: 16:00
Regular seats	15
Course fee	11,280.00 kr.
Lecturers	Kristine Freude
ECTS credits	4.10

Contact person	Betina Wingreen Jensen E-mail address: bwj@sund.ku.dk

Enrolment Handling/Course Organiser	PhD administration E-mail address: phdkursus@sund.ku.dk

Aim and content This course is free of charge for PhD students at Danish universities (except Copenhagen Business School), and for PhD students at graduate schools in the other Nordic countries. All other participants must pay the course fee. Anyone can apply for the course, but if you are not a PhD student at a Danish university, you will be placed on the waiting list until enrollment deadline. This also applies to PhD students from Nordic countries. After the enrollment deadline, available seats will be allocated to applicants on the waiting list. Learning objectives A student who has met the objectives of the course will be able to: 1. • Freeze and thaw human induced pluripotent stem cells 2. • Maintain and expand human induced pluripotent stem cells 3. • Generate iPSC via electroporation of episomal plasmids 4. • Differentiate human induced pluripotent stem cells into astrocytes, glia and cortical neurons 5. • Generate 3D cultures 6. • Analyze induced pluripotent stem cell-derived neural cells using different molecular methodologies 7. • Design CRISPRs guides and templates for gene-editing of pluripotent stem cells 8. • Perform nucleofection based CRISPR-Cas9 gene editing 9. • Acquire a detailed understanding of the derivation of pluripotent stem cells 10. • Acquire a detailed theoretical understanding of the states of pluripotency 11. • Acquire a detailed understanding of early neural developmental processes and molecular cues that govern neural patterning 12. • Acquire a detailed understanding of the latest protocols for differentiation of iPSC into neural subtypes, astrocytes and microglia 13. • Gain detailed knowledge on state-of-the-art methods for culturing pluripotent stem cells 14. • Gain detailed knowledge on the latest methods for differentiating pluripotent stem cells into neural cell types (with focus on neurons, astrocytes) in 2D and 3D 15. • Gain detailed knowledge on the latest methods for differentiating pluripotent stem cells into microglia 16. • Gain expertise on use and application of CRISPR guides in gene-editing of pluripotent stem cells 17. • Will be able to develop custom-designed experiments in their own lab pertaining to pluripotent stem cell culture and differentiation into neural cells, glia and microglia 18. • Will be able to custom-design CRISPRs for gene-editing of their own cell lines 19. • Will be able to perform iPSC reprogramming and CRISPR-Cas9 gene editing using electroporation based methods Content The course includes the following topics: Pluripotent stem cell biology, Neural development and molecular patterning of the developing brain, Neural differentiation, Astrocyte differentiation, Microglia differentiation, Tissue culture of pluripotent stem cells, Tissue culture of different neural subtypes, astrocytes, 3D cultures, glia and microglia, CRISPR-Cas9 technologies and applications in pluripotent stem cells Participants PhD students, postdoctoral researchers and technicians embarking on culture of pluripotent stem cells with application for neural cell studies, microglia and optic cups Relevance to graduate programmes The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH: - All graduate programmes - Neuroscience - Molecular Mechanisms of Disease Language English Form Lectures, keynote lectures, group exercises, practical laboratory work, student presentations Course director Kristine Freude, Associate Professor, Dept. of Veterinary Clinical and Animal Sciences, kkf@sund.ku.dk Teachers Kristine Freude, Associate Professor, University of Copenhagen Stanislava Pankratova, Assistant Prof, University of Copenhagen Agnete Kirkeby, Associate Professor, University of Copenhagen Blanca Aldana, University of Copenhagen Morten Meyer, SDU Ludo van Den Bosch, Leuven University Eric Bennett, Novo Nordisk A/S Dates May 6-12, 2022 Course location Frederiksberg Campus, Grønnegårdsvej 7 Registration Please register before April 6, 2022 Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules. Applications from other participants will be considered after the last day of enrolment. Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.

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