Graduate School of health and Medical Sciences

Induced Pluripotent Stem Cells, Gene-editing and Brain Cell Models
Provider: Faculty of Health and Medical Sciences

Activity no.: 3630-23-00-00	There are no available seats
	Enrollment deadline: 19/03/2023

Date and time	17.04.2023, at: 08:30 - 21.04.2023, at: 16:30
Regular seats	18
Course fee	11,280.00 kr.
Lecturers	Kristine Freude
ECTS credits	4.10

Contact person	Betina Wingreen Jensen E-mail address: bwj@sund.ku.dk

Enrolment Handling/Course Organiser	PhD administration E-mail address: phdkursus@sund.ku.dk

Aim and content This course is free of charge for PhD students at Danish universities (except Copenhagen Business School), and for PhD Students from NorDoc member universities. All other participants must pay the course fee. Anyone can apply for the course, but if you are not a PhD student at a Danish university, you will be placed on the waiting list until enrollment deadline. This also applies to PhD students from NorDoc member universities. After the enrollment deadline, available seats will be allocated to applicants on the waiting list. Learning objectives A student who has met the objectives of the course will be able to: Skills: • Freeze and thaw human induced pluripotent stem cells • Maintain and expand human induced pluripotent stem cells • Generate iPSC via electroporation of episomal plasmids • Differentiate human induced pluripotent stem cells into astrocytes, microglia, and cortical neurons • Generate 3D cultures • Analyze induced pluripotent stem cell-derived neural cells using different molecular methodologies • Design CRISPRs guides and templates for gene-editing of pluripotent stem cells • Perform nucleofection based CRISPR-Cas9 gene editing Knowledge: • Acquire a detailed understanding of the derivation of pluripotent stem cells • Acquire a detailed theoretical understanding of the states of pluripotency • Acquire a detailed understanding of early neural developmental processes and molecular cues that govern neural patterning • Acquire a detailed understanding of the latest protocols for differentiation of iPSC into neural subtypes, astrocytes, and microglia • Gain detailed knowledge on state-of-the-art methods for culturing pluripotent stem cells • Gain detailed knowledge on the latest methods for differentiating pluripotent stem cells into neural cell types (with focus on neurons, astrocytes) in 2D and 3D • Gain detailed knowledge on the latest methods for differentiating pluripotent stem cells into microglia • Gain expertise on use and application of CRISPR guides in gene-editing of pluripotent stem cells Competences: • Will be able to develop custom-designed experiments in their own lab pertaining to pluripotent stem cell culture and differentiation into neural cells, glia and microglia • Will be able to custom-design CRISPRs for gene-editing of their own cell lines • Will be able to perform iPSC reprogramming and CRISPR-Cas9 gene editing using electroporation-based methods Content Pluripotent stem cell biology, Neural development and molecular patterning of the developing brain, Neural differentiation, Astrocyte differentiation, Microglia differentiation, Tissue culture of pluripotent stem cells, Tissue culture of different neural subtypes, astrocytes, 3D cultures, glia, and microglia, CRISPR-Cas9 technologies and applications in pluripotent stem cells Participants PhD students, postdoctoral researchers and technicians embarking on culture of pluripotent stem cells with application for neural cell studies, microglia, and optic cups Relevance to graduate programs The course is relevant to PhD students from the following graduate programs at the Graduate School of Health and Medical Sciences, UCPH; Neuroscience Academy Denmark (https://neuroscienceacademydenmark.dk/) All graduate programs Neuroscience Molecular Mechanisms of Disease Language English Form Lectures, keynote lectures, group exercises, practical laboratory work, student presentations Course director Kristine Freude, Associate Professor, Dept. of Veterinary Clinical and Animal Sciences, kkf@sund.ku.dk Teachers Agnete Kirkeby, Associate Professor, University of Copenhagen Blanca Aldana, Associate Professor, University of Copenhagen Kristine Freude, Professor, University of Copenhagen Stanislava Pankratova, Associate Professor, University of Copenhagen Jan Gorodkin, Professor, University of Copenhagen Morten Meyer, Professor, University of Southern Denmark Andras Dinnyés, BioTalentum, Hungary Dates April 17-21, 2023 Course location Frederiksberg Campus, Grønnegårdsvej 7 Registration Please register before March 19, 2023 Expected frequency yearly Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules. Applications from other participants will be considered after the last day of enrollment. Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.

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