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Signalling in Embryonic Development and Stem Cells
Provider: Faculty of Health and Medical Sciences
Activity no.: 3243-15-00-00
Enrollment deadline: 15/09/2015
Date and time
09.11.2015, at: 09:00 - 13.11.2015, at: 18:00
Regular seats
12
Course fee
3,000.00 kr.
Lecturers
Palle Serup
Elke Ober
ECTS credits
4.50
Contact person
Anne Vognsen E-mail address: anne.vognsen@bric.ku.dk
Enrolment Handling/Course Organiser
PhD administration E-mail address: phdkursus@sund.ku.dk
Aim and content
Learning objectives
A student who has met the objectives of the course will be able to:
a. Describe key developmental signalling pathways and their interactions.
b. Explain commonalities and differences between embryonic development and stem cells.
c. Plan, conduct, analyse, and draw conclusions from gain- and loss-of-function experiments in embryos and stem cells.
d. Handle, differentiate, and analyse embryonic stem cells.
e. Handle zebrafish embryos and analyse developmental phenotypes.
Content
Theoretical introduction of three key signalling pathways acting in embryos and stem cells (Wnt, Bmp and Nodal).
Introduction to basic concepts of stem cell derivation, differentiation potential and ethics.
Practical introduction into embryonic stem cells (ESCs):
? Hands-on experience w. handling of cells in the laminar flow-hood.
? Differentiation of ESCs into tissue specific fates (embryoid bodies) by culturing with agonists and antagonists of signalling.
? Analysing and evaluating results, drawing conclusions and planning possible follow-up experiments.
Practical introduction into zebrafish embryos:
? Hands-on experience w. handling of zebrafish embryos.
? Testing roles of signalling pathways in embryonic development by treatment with agonists and antagonists of signalling.
? Analysing and evaluating results, drawing conclusions and planning possible follow-up experiments.
Participants
12 students minimum - 12 students maximum ; prior experience with cell culture and a basic understanding of embryology and gastrulation is a major advantage.
Language
English (main language; clarifications in Danish are possible).
Form
Lectures (planned are 3 main lectures from speakers abroad).
Presentations introducing the theory of the experimental work.
Practical laboratory experiments - working in teams of 2 students and small groups
Presentation of results.
Hours: 9 – 18 Monday to Friday
Course director
Palle Serup, Professor, DanStem University of Copenhagen palle.serup@sund.ku.dk
Elke Ober, Associate Professor, DanStem University of Copenhagen elke.ober@sund.ku.dk
Teachers
Mette C. Jørgensen, Assistant Professor, DanStem, University of Copenhagen.
Gelo Dela Cruz, FACS specialist, DanStem, University of Copenhagen.
2-4 additional teaching assistants to be named closer to the starting date.
Course secretary
Anne Vognsen
Dates
09 – 13 November 2015
Course location
DanStem (Panum bygning 6, 4.tv)
Registration: Please register before 30 June 2015 (possibility to extend until mid-August)
Admission to PhD students from Danish universities will be allocated on a first-come, first-served basis and according to the rules in force.
Applications from other participants will be considered after the last day of enrolment.
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