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Advanced flow cytometry II
Provider: Faculty of Health and Medical Sciences

Activity no.: 3216-20-00-00There are no available seats 
Enrollment deadline: 01/02/2020
Date and time17.02.2020, at: 09:00 - 28.04.2020, at: 16:00
Regular seats12
Course fee12,960.00 kr.
LecturersJan Pravsgaard Christensen
ECTS credits5.40
Contact personJan Pravsgaard Christensen    E-mail address:  jpc@sund.ku.dk
Enrolment Handling/Course OrganiserPhD administration     E-mail address: fak-phdkursus@sund.ku.dk

Aim and content
This course is free of charge for PhD students at Danish universities (except Copenhagen Business School), and for PhD students at graduate schools in the other Nordic countries. All other participants must pay the course fee.

Anyone can apply for the course, but if you are not a PhD student at a Danish university, you will be placed on the waiting list until enrollment deadline. This also applies to PhD students from Nordic countries. After the enrollment deadline, available seats will be allocated to applicants on the waiting list.


Learning objectives
A student who has met the objectives of the course will be able to:

1. Optimize staining of a sample and settings for the instrument before running samples

2. Design and make an instrument dependent spread matrix. Use the spread matrix for design of multicolor flow panels

3. Understand the standards of publishing and storing flow cytometry related data

4. Analyze and interpret data based on tSNE and FlowSOM software

5. Understand the principle of barcoding in flow cytometry

6. Design a multicolor experiment with relevant controls, fit for interpretation and publication


Content
The course is divided in 5 themes, each covering part of a flow cytometer experiment. We will take you through design of multi-color panels, how to get the best sensitivity on your measurements, what controls to pick for the experiment, and how to analyze/evaluate the experiment. We will also show you how barcoding of samples can optimize the time spend at the instruments and minimize variation of samples that have been taken at different time points e.g. kinetic studies in patients.

The course will include the use of FlowLogic and FlowJo for analyzing samples, as well as tSNE and FlowSOM for analysis and generation of hypothesis.


Participants
PhD students and people on similar level.

We recommend that you have had our Basic course in flow cytometry and cell sorting or a similar course, and we recommend that you have work with multicolor flow cytometry for a year, to get the most out of the course.

There will be work on different instruments and software, and there will not be time to train people in basic use of an instrument and knowledge about flow cytometry.


Relevance to graduate programmes
The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH:

Immunology and Infectious Diseases


Language
English


Form
Each theme is a 2-week block where students have to watch some e-learning videos and read some papers to prepare for the practical part. In each theme, we will meet for 1 or 2 days to make practical and/or theoretical exercises, related to the theme.


Course director
Professor Jan Pravsgaard Christensen, ISIM, 35327873, jpc@sund.ku.dk


Teachers
Jan Pravsgaard Christensen, Professor, PhD, postdocs, and technicians at the department


Dates
17-28 Feb, home studies to prepare for exercise, app 2-3 hours

3 Mar, 9-16, exercise

4-13 Mar, home studies to prepare for exercise, app 2-3 hours

17 Mar, 9-16, exercise

18 Mar, 9-16, exercise

19-27 Mar, home studies to prepare for exercise, app 2-3 hours

31 Mar, 9-16, exercise

1-10 Apr, home studies to prepare for exercise, app 2-3 hours

14 Apr, 9-16, exercise

15-24 Apr, home studies to prepare for exercise, app 2-3 hours

28 Apr, 9-16, exercise


Course location
The Panum Institute, Blegdamsvej 3B, DK-2200 Copenhagen N. Maersk Tower, 14th floor.


Registration
Please register before 1 February 2020

Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules.
Applications from other participants will be considered after the last day of enrolment.

Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.

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