Graduate School of health and Medical Sciences

Basic flow cytometry and cell sorting I
Provider: Faculty of Health and Medical Sciences

Activity no.: 3215-24-00-02
	Enrollment deadline: 11/08/2024

Date and time	09.09.2024, at: 09:00 - 27.09.2024, at: 16:00
Regular seats	12
Course fee	12,240.00 kr.
Lecturers	Jan Pravsgaard Christensen
ECTS credits	5.10

Contact person	Jan Pravsgaard Christensen E-mail address: jpc@sund.ku.dk

Enrolment Handling/Course Organiser	PhD administration E-mail address: phdkursus@sund.ku.dk

Aim and content This course is free of charge for PhD students at Danish universities (except Copenhagen Business School), and for PhD Students from NorDoc member faculties. All other participants must pay the course fee. Anyone can apply for the course, but if you are not a PhD student at a Danish university, you will be placed on the waiting list until enrollment deadline. This also applies to PhD students from NorDoc member faculties. After the enrollment deadline, available seats will be allocated to applicants on the waiting list. Learning objectives A student who has met the objectives of the course will be able to: 1. The student will be able to identify, relevant protocols to analyze the samples with, define relevant controls needed for analysis, understand the limitation of flow cytometry, and be able to analyze obtained data in terms of publication/scientific presentation 2. The student will be able to plan their experimental setup for running and analyzing flow cytometry samples, apply this in a practical setting, analyze the achieved data, and compare the data to other groups. 3. The student will be able to run simple samples/protocols on flow cytometry and cell sorter, evaluate presented data from flow cytometry, and discuss flow cytometry on a basic level. Content The course will take you through basic knowledge of how to design and choose the right experiment, how to operate the instrument, and some of the pitfalls in flow cytometry. Several techniques will be demonstrated and the student will get a chance to work with these on our different instruments (FACSCalibur, Fortessa, and Aria-II). The course is focused on the use of diva software, and to some extent CellQuest because of our choice of instruments, but independent software such as FlowLogic will also be demonstrated. During the course some exercises will require preparation from day to day, since presentation / discussion with the group is needed. Participants PhD students and people on similar level, no previous skills in flow cytometry are needed. Relevance to graduate programmes The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH: Immunology and Infectious Diseases Language English Form E-learning lectures, practical and theoretical exercises. The course has a follow up where students can get help designing their own experiments. Course director Professor Jan Pravsgaard Christensen, ISIM, 35327873, jpc@sund.ku.dk Teachers Jan Pravsgaard Christensen, Professor, PhD and postdocs/technicians at the institute Dates 9 – 23 September 2024, you are expected to watch e-learning videos at home, and test yourself, app. 30 hours 24 September 2024: 9-13 practical exercise in core facility 25 September 2024: 9-12 practical exercise in core facility 26 September 2024: 9-12 practical exercise in core facility 27 September 2024: 9-16 practical exercise with data analysis in core facility Course location The Panum Institute, Blegdamsvej 3B, DK-2200 Copenhagen N. Maersk Tower, 14th floor. Registration Please register before 11 August 2024 Expected frequency We have 4 courses a year, end January, end February, end September, and end October. Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules. Applications from other participants will be considered after the last day of enrolment. Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.

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